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Thromb Haemost 1962; 08(03): 472-484
DOI: 10.1055/s-0038-1655444
Originalarbeiten — Original Articles — Travaux Originaux
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The Nature of the Thromboplastic Activity Appearing during the Clotting of Pig’s Plasma

M. J Cross
1   Department of Pharmacology, Institute of Basic Medical Sciences, Royal College of Surgeons of England, London WC 1
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Publication Date:
14 May 2019 (online)

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Summary

1 During clotting, pigs’ plasma developed thromboplastic activity. This activity was compared with that of thrombin in accelerating the clotting of platelet-deficient plasma and of fibrinogen in the presence and absence of calcium.

2. The thromboplastic activity of the plasma could be mimicked by a solution of thrombin of the right strength.

3. The thromboplastic activity could be stabilised by freezing but disappeared rapidly at 37° C. Thrombin added to the serum was inactivated at almost exactly the same rate; and the rates of loss of both activities were considerably reduced by cooling, by decalcification with sodium ethylene-diaminetetraacetate, and by acidification.

4. It is concluded that the thromboplastic activity of undiluted recalcified pigs’ plasma can be accounted for by its thrombin content alone. It also seems likely that the activity measured in the screening test for disorders of thromboplastin generation described by Hicks and Pitney (1957) can be attributed wholly to thrombin.

 

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