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Thromb Haemost 1962; 08(03): 434-441
DOI: 10.1055/s-0038-1655441
Originalarbeiten — Original Articles — Travaux Originaux
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Procedures for the Quantitative Determination of Autoprothrombin C[*]

Edmond R Cole
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
,
Ewa Marciniak   **
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
,
Walter H Seegers
1   Department of Physiology and Pharmacology Wayne State University, College of Medicine, Detroit, Michigan, USA
› Author Affiliations
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Publication Date:
14 May 2019 (online)

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Summary

Two quantitative procedures for autoprothrombin C are described. In one of these purified prothrombin is used as a substrate, and the activity of autoprothrombin C can be measured even if thrombin is in the preparation. In this procedure a reaction mixture is used wherein the thrombin titer which develops in 20 minutes is proportional to the autoprothrombin C in the reaction mixture. A unit is defined as the amount which will generate 70 units of thrombin in the standardized reaction mixture. In the other method thrombin interferes with the result, because a standard bovine plasma sample is recalcified and the clotting time is noted. Autoprothrombin C shortens the clotting time, and the extent of this is a quantitative measure of autoprothrombin C activity.

* This investigation was supported by a research grant HE-05141 from the National Heart Institute, National Institutes of Health, U. S. Public Health Service.


** Rockefeller Foundation Research Fellow.


 

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