Thromb Haemost 1962; 08(01): 101-111
DOI: 10.1055/s-0038-1655416
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

On the Inhibiting Effect of ε-Amino Caproic Acid upon the Fibrinolytic System During „Spontaneous Activation”

W Doleschel
1   Department of Physiology (Director: Prof. G. Schubert) University of Vienna, School of Medicine
,
W Auerswald
1   Department of Physiology (Director: Prof. G. Schubert) University of Vienna, School of Medicine
,
A von Lützow
1   Department of Physiology (Director: Prof. G. Schubert) University of Vienna, School of Medicine
› Author Affiliations
Further Information

Publication History

Publication Date:
14 May 2019 (online)

Summary

The effect of ε-ACA (ε-amino caproic acid) upon the factors of the fibrinolytic system contained in a human euglobulin preparation was studied during “spontaneous activation“. The results obtained with the bovine fibrin plate method, the clot lysis, and the casein tests demonstrated a strong inhibitory effect of ε-ACA on the formation of a serum plasminogen activator and on the conversion of plasminogen into plasmin by this activator resulting in a “potentiated inhibition“. On the other hand, no inhibition of plasmin could be found with ε-ACA in concentrations below 0.1 molar. In tests on human fibrin plates finally it became obvious that the human serum plasminogen activator contained in purified human plasmin preparations, having a much higher specificity towards the human plasminogen than the bovine one becomes inhibited by relatively low ε-ACA concentrations. The significance of these results for the inhibitory effect of ε ACA on the fibrinolytic system in vivo was discussed.

 
  • References

  • 1 Abe T, Sato A. Reaction Mechanism between e-Aminocaproic Acid and Plasmin, Tissue Activator and Trypsin.. Keio J. Med. 1959; 8: 219
  • 2 Ablondi F. B, Hagan J. J, Philips M, de Renzo E. C. Inhibition of Plasmin, Trypsin and the Streptokinase-Activated Fibrinolytic System by e-Aminocaproic Acid.. Arch. biochem. 1959; 82: 153
  • 3 Alkjaersig N, Fletcher A. P, Sherry S. e-Aminocaproic Acid: an Inhibitor of Plasminogen Activation.. J. biol. Chem. 1959; 234: 832
  • 4 Auerswald W, Doleschel W. Zur Frage der Beständigkeit humaner fibrinolytisch aktiver Substanzen bei neutralen pH in Abhängigkeit von der Ionenstärke.. Med. exp. 1960; 2: 42
  • 5 Auerswald W, Doleschel W. Critical Evaluation of the Fibrin Plate Method for Estimating Fibrinolytic Enzymes and Activators.. Thrombos. Diathes. haemorrh. 1960; 1: 112
  • 6 Doleschel W, Auerswald W. Untersuchungen über die Spontanaktivierung von humanem Plasminogen in Gegenwart hoher Salzkonzentrationen.. Med. exp. 1960; 2: 339
  • 7 McNicol G. P, Fletcher A. P, Alkjaersig N, Sherry S. The Absorption, Distribution and Excretion of e-Aminocaproic Acid following Oral or Intravenous Administration to Man.. J. Lab. clin. Med. 1962; 59: 15
  • 8 Nilsson I. M, Sjoerdsma A, Waldenström J. Antifibrinolytic Activity and Metabolism of e-Aminocaproic Acid in Man.. Lancet I: 1322 1960;
  • 9 Remmert L. F, Cohen P. P. Partial Purification and Properties of a Proteolytic Enzyme of Human Serum.. J. biol. Chem. 1949; 181: 431