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Thromb Haemost 1967; 18(03/04): 592-604
DOI: 10.1055/s-0038-1655068
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

An Electron Microscopic Study of Platelet Thrombus Formation in the Rabbit with Particular Regard to 5-Hydroxytryptamine Release[*]

H. R Baumgartner
1   Department of Experimental Medicine, F. Hoffmann-La Roche and Co., Ltd, Basle, Switzerland
,
J. P Tranzer
1   Department of Experimental Medicine, F. Hoffmann-La Roche and Co., Ltd, Basle, Switzerland
,
A Studer
1   Department of Experimental Medicine, F. Hoffmann-La Roche and Co., Ltd, Basle, Switzerland
› Author Affiliations
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Publication History

Publication Date:
26 June 2018 (online)

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Summary

Electron microscopic and histologic examination of rabbit ear vein segments 4 and 30 min after slight endothelial damage have yielded the following findings :

1. Platelets do not adhere to damaged endothelial cells.

2. If the vessel wall is denuded of the whole endothelial cell, platelets adhere to the intimai basement lamina as do endothelial cells.

3. The distance between adherent platelets as well as endothelial cells and intimai basement lamina measures 10 to 20 mµ, whereas the distance between aggregated platelets is 30 to 60 mµ.

4. 5-hydroxytryptamine (5-HT) is released from platelets during viscous metamorphosis at least in part as 5-HT organelles.

It should be noted that the presence of collagen fibers is not necessary for platelet thrombus formation in vivo.

* Herrn Prof. A. Werthemann zum 70. Geburtstag gewidmet.


 

  • References

  • 1 Ashford T. P, Freiman D. G. The role of the endothelium in the initial phases of thrombosis. An electron microscopic study. Amer. J. Path. 50: 257-274 1967;
    PubMedSearch in Google Scholar
  • 2 Baumgartner H. R, Studer A, Heber K. Über Methoden zur Erzeugung experimenteller Thrombosen am Kaninchenohr und die Bedeutung von 5-Hydroxytryptamin bei deren Entstehung. Thrombos. Diathes. haemorrh. (Stuttg) 09: 485-511 1963;
    PubMedSearch in Google Scholar
  • 3 Born G. V. R. The effects of substances related to adenosine on blood vessels and on blood platelets. Vorträge des 4. Freiburger Kolloquiums über Kreislaufmessungen, 4 06: 4 1963;
    PubMedSearch in Google Scholar
  • 4 Eberth J. G, Schimmelbusch G. Experimentelle Untersuchungen über Thrombose. Virchows Arch. path. Anat. 103: 39-87 1886;
    CrossrefPubMedSearch in Google Scholar
  • 5 Erichson R. B, Katz A. J, Cintron J. R. Ultrastructural observations on platelet adhesion reactions. I. Platelet-fibrin interaction. Blood 29: 385-400 1967;
    PubMedSearch in Google Scholar
  • 6 French J. E, Macfarlane R. G, Sanders A. G. The structure of haemostatic plugs and experimental thrombi in small arteries. Brit. J. exp. Path. 45: 467-474 1964;
    PubMedSearch in Google Scholar
  • 7 Grette K. Studies on the mechanism of thrombin-catalyzed hemostatic reactions in blood platelets. Acta physiol. scand. 56 Suppl. 195 1962;
    PubMedSearch in Google Scholar
  • 8 Hovig T. Release of a platelet-aggregating substance (adenosine diphosphate) from rabbit blood platelets induced by saline “extract” of tendons. Thrombos. Diathes. haemorrh. (Stuttg) 09: 248-263 1963;
    PubMedSearch in Google Scholar
  • 9 Hugues J. Accollement des plaquettes au collagène. C. R. Soc. Biol. (Paris) 154: 866-868 1960;
    PubMedSearch in Google Scholar
  • 10 Kjaerheim A, Hovig T. The ultrastructure of haemostatic blood platelet plugs in rabbit mesenterium. Thrombos. Diathes. haemorrh. (Stuttg) 07: 1-15 1962;
    PubMedSearch in Google Scholar
  • 11 Luft J. H. Fine structure of capillary and endo-capillary layer as revealed by ruthenium red. Fed. Proc. 25: 1773-1783 1966;
    PubMedSearch in Google Scholar
  • 12 Marcus A. J, Zucker M. B. The physiology of blood platelets. Grune & Stratton: New York; 1965
    Search in Google Scholar
  • 13 Matthews M. A, Gardner D. L. The fine structure of the mesenteric arteries of the rat. Angiology 17: 902-928 1966;
    CrossrefPubMedSearch in Google Scholar
  • 14 Spaet T. H, Zucker M. B. Mechanism of platelet plug formation and role of adenosine diphosphate. Amer. J. Physiol. 206: 1267-1274 1964;
    PubMedSearch in Google Scholar
  • 15 Studer A. Experimental platelet thrombus. In: Koller F, Duckert F, Streuli F. Pathogenesis and treatment of thromboembolic diseases. 109-130 Schattauer; Stuttgart: 1966
    Search in Google Scholar
  • 16 Tranzer J. P, Da Prada M, Pletscher A. Ultrastructural localization of 5-hydroxy thryp ta- mine in blood platelets. Nature (Lond) 212: 1574 1966;
    CrossrefPubMedSearch in Google Scholar
  • 17 Weibel E. R, Palade G. E. New cytoplasmatic components in arterial endothelia. J. Cell. Biol 23: 101-112 1964;
    CrossrefPubMedSearch in Google Scholar