RSS-Feed abonnieren
PDF herunterladen
DOI: 10.1055/s-0038-1655039
The Immunological Properties of Factor VIII II. The Immunoelectrophoretic Studies
Publikationsverlauf
Publikationsdatum:
26. Juni 2018 (online)
Summary
The results of immunoelectrophoretic studies with, the use of antisera against factor VIII preparations have been presented. When normal or hemophilic plasmas were analysed with the anti-I-0 serum 7 precipitation lines were obtained. Six of them were identified as albumin, alpha 2 macroglobulin, beta lipoprotein, fibrinogen, IgM and IgG globulins. One unidentified line found in betax globulin region seems to have some relation to factor VIII. This precipitation line was absent when plasma from one patient with anti-AHG inhibitor and normal plasma adsorbed on bentonite were studied. It was very faint in ief patterns of von Willebrand’s disease plasma. The possibility of 2 kinds of antibodies directed against 2 hypothetical sub-units of factor VIII is discussed.
-
References
- 1 Adelson E, Rheingold J, Parker O, Steiner M, Kirby J. G. The survival of factor VIII (antihemophilic globulin) and factor IX (plasma thromboplastin component) in normal humans. J. clin. Invest 42: 1040 1963;
- 2 Berglund G. Immunological studies of haemophilic plasma. Int. Arch. Allergy 22: 1 1963;
- 3 Biggs R, Macfarlane R. G. Human blood coagulation and its disorders. 3rd. ed.. Blackwell Oxford: 1962
- 4 Bidwell E. Immunological aspects of haemophilia in Treatment of haemophilia and other coagulation disorders. Edited by Biggs R, Macfarlane R. G. 93 Blackwell, Oxford: 1966
- 5 Graham J. B. The genetics of hemophilia. Scand. J. Haemat., Series Haematologica 07: 14 1965;
- 6 Heremans I. Les globulines sériques du système gammaleur nature et leur pathologie. Masson, Paris. Arsica, Bruxelles; 1961
- 7 McLester W. D, Wagner R. H. Antibody to antihemophilic factor and its lack of reaction with hemophilic plasma. Amer. J. Physiol. 208: 499 1965;
- 8 Richards M. D, Spaet T. H. Immunization of rabbits against human antihemophilic factor. Blood 11: 473 1956;
- 9 Scheidegger J. J. Une micro-méthode de l’immuno-electrophorése. Int. Arch. Allergy 07: 103 1955;
- 10 Shanberge J. N, Gore I. Studies on the immunologic and physiologic activities of antihemophilic factor (AHF). J. Lab. clin. Med. 50: 945 1957;
- 11 Uszynski L. The immunological properties of factor VIII. I. Studies on the inhibitory activity of specific antisera to human antihemophilic globulin. Thrombos. Diathes. haemorrh. (Stuttg) 14: 559 1966;