Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1967; 18(01/02): 161-166DOI: 10.1055/s-0038-1655025 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Inhibitor of Blood Clotting Derived from Prothrombin[*] Ewa Marciniak 1 Department of Physiology and Pharmacology, Wayne State University, Detroit, Michigan , Genesio Murano 1 Department of Physiology and Pharmacology, Wayne State University, Detroit, Michigan , Walter H Seegers 1 Department of Physiology and Pharmacology, Wayne State University, Detroit, Michigan› Author AffiliationsRecommend Article Abstract Buy Article Summary Purified prothrombin was dissociated with thrombin and one of the three main degradation products was separated as a protein and found to be a competitive inhibitor of autoprothrombin C. Relatively large amounts were needed to inhibit the generation of thrombin activity. This slowing of thrombin generation occurred whether prothrombin or prethrombin was the source of thrombin. The main evidence is consistent with the view that the inhibitor originates from the prethrombin portion of prothrombin. Full Text References References 1 Mammen E. F, Thomas W. R, Seegers W. H. Activation of purified prothrombin to autoprothrombin I or autoprothrombin II (platelet cofactor II) or autoprothrombin II-A. Thrombos. Diathes. haemorrh. (Stuttg) 05: 218 1960; 2 Seegers W. H, Heene D. L, Marciniak E. Activation of purified prothrombin in ammonium sulfate solutions: Purification of autoprothrombin C. Thrombos. Diathes. haemorrh. (Stuttg) 15: 1 1966; 3 Seegers W. H, Ulutin O. N. Autoprothrombin II-anticoagulant (autoprothrombin II-A. Thrombos. Diathes. haemorrh. (Stuttg) 06: 270 1961; 4 Seegers W. H, Marciniak E. Some activation characteristics of the prethrombin subunit of prothrombin. Life Sciences 04: 1721 1965; 5 Seegers W. H. The purification of prothrombin. Rec. Chem. Progr. 13: 143 1952; 6 Seegers W. H, Levine W. G, Shepard R. S. Further studies on the purification of thrombin. Canad. J. Biochem. 36: 603 1958; 7 Hecht E. R, Gho M. H, Seegers W. H. Thromboplastin: Nomenclature and preparations of protein-free material different from platelet factor 3 or lipid activation. Amer. J. Physiol. 193: 854 1958; 8 Ware A. G, Seegers W. H. Two-stage procedure for the quantitative determination of prothrombin concentration. Amer. J. clin. Path. 19: 471 1949; 9 Seegers W. H, Smith H. P. Factors which influence the activity of purified thrombin. Amer. J. Physiol. 137. 348 1942 10 Cole E. R, MarciniaJc E, Seegers W. H. Procedure for the quantitative determination of autoprothrombin C. Thrombos. Diathes. haemorrh. (Stuttg) 08: 434 1962; 11 Langdeil R. D, Wagner R. H, Brinkhous K. M. Effect of antihemophilic factor on one-stage clotting test. A presumptive test for hemophilia and a simple one-stage antihemophilic factor assay procedure. J. Lab. clin. Med. 41: 637 1953; 12 Macfarlane R. G. An enzyme cascade in blood clotting mechanism, and its functions as a biochemical amplifier. Nature (Lond) 202: 498 1964; 13 Davie E. W, Ratnojf O. D. Waterfall sequence for intrinsic blood clotting. Science 145: 1310 1964; 14 Alexander B, Goldstein R, Landwehr G, Cook G. D. Congenital S.P.C. A. deficiency: A hitherto unrecognized coagulation defect with hemorrhage rectified by serum and serum fractions. J. clin. Invest 30: 596 1951; 15 Biggs R, Douglas A. S, Macfarlane R. G, Dacie J. V, Pitney W. R, Merskey C, O’Brien J. R. Christmas disease: A condition previously mistaken for haemophilia. Brit. med. J. II: 1378 1952; 16 Seegers W. H. Prothrombin. Harvard University Press; Cambridge, Massachusetts: 1962 17 Seegers W. H. Basic enzymology of blood coagulation. Thrombos. Diathes. haemorrh. (Stuttg) 14: 213 1965; 18 Marciniak E, Seegers W. H. Experiments with prothrombin, prethrombin, autoprothrombin III and plasmas with prothrombin related deficiencies. New Istanbul Contr. clin. Sci. 08: 117 1965; 19 Lineweaver H, Burk D. The determination of enzyme dissociation constants. J. Amer, chem. Soc. 56: 658 1934; 20 Milstone J. H. Prothrombokinase and the three stages of blood coagulation. Science 106: 546 1947;
