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Thromb Haemost 1964; 11(01): 064-074
DOI: 10.1055/s-0038-1654806
Originalarbeiten — Original Articles — Travaux Originaux
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Purification of Antihemophilic Factor (Factor VIII) by Amino Acid Precipitation*

Robert H Wagner   **
1   Departments of Pathology and Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina
,
William D McLester
1   Departments of Pathology and Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina
,
Marion Smith
1   Departments of Pathology and Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina
,
K. M Brinkhous
1   Departments of Pathology and Biochemistry, University of North Carolina School of Medicine, Chapel Hill, North Carolina
› Author AffiliationsThis investigation was aided by a grant (H-1648) from the National Heart Institute of the National Institutes of Health, Public Health Service.
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

1. The use of several amino acids, glycine, alpha-aminobutyric acid, alanine, beta-alanine, and gamma-aminobutyric acid, as plasma protein precipitants is described.

2. A specific procedure is detailed for the preparation of canine antihemophilic factor (AHF, Factor VIII) in which glycine, beta-alanine, and gammaaminobutyric acid serve as the protein precipitants.

3. Preliminary results are reported for the precipitation of bovine and human AHF with amino acids.

** This investigator was supported by a Public Health Service research career program award (K3-GM-1147).


 

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