Changes Occurring During Coagulation in Glass. I. Normal Human Blood^[*)]

Summary

Normal whole blood was allowed to stand in glass tubes at 37° C, and the clotting process stopped at various intervals by the addition of sodium oxalate. During the first 15 minutes a marked acceleration of clotting activity was found. Study of the individual coagulation factors showed the following changes: a sustained and rapid fall in platelet count, a sustained and rapid rise in PTC (factor IX), a steady fall in fibrinogen, a more gradual fall in AHF (factor VIII), a rapid rise and subsequent fall in proaccelerin (factor V) activity, a somewhat lesser and slower rise and fall in proconvertin (factor VII) activity, and a slow fall in prothrombin concentration. No changes were noted in Hageman factor or PTA activities.

^*) Supported in part by research grants (H-2254, H-1510) from the Division of Research Grants of the National Institutes of Health, U. S. Public Health Service.

^**) Part of this investigation was carried out during the tenure of a Life Insurance Fellowship.

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