Thrombin Formation in Purified Systems

Douglas M Surgenor; Barbara B Steele; Donald F. H Wallach

doi:10.1055/s-0038-1654383

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1959; 03(02): 214-226
DOI: 10.1055/s-0038-1654383

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Thrombin Formation in Purified Systems

Douglas M Surgenor

¹Department of Biological Chemistry, Harvard Medical School and Protein Foundation, Boston, Mass., USA

,

Barbara B Steele

¹Department of Biological Chemistry, Harvard Medical School and Protein Foundation, Boston, Mass., USA

,

Donald F. H Wallach

¹Department of Biological Chemistry, Harvard Medical School and Protein Foundation, Boston, Mass., USA

› Author Affiliations

Abstract

Summary

Study of the kinetic behavior of purified human clotting factors suggests that formation of prothrombin activator from platelets, plasma thromboplastin component (PTC), antihemophilic factor (AHF), Factor V and calcium proceeds by a sequence of reactions. The earliest detectable reaction is slow and involves platelets, PTC and calcium; an intermediate activated PTC is obtained which is free of platelet contamination and is characterized by its kinetic behavior. Platelets are also requisite at a later step in the sequence, possibly in an intermediate formed between activated PTC, AHF, platelets and calcium. Variations in platelets, AHF and PTC result in characteristic responses in the kinetics of thrombin formation.

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References

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