Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1971; 25(01): 105-113DOI: 10.1055/s-0038-1654284 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Large-Scale Production of Intermediate-Purity, Soluble Human Plasminogen[*] K Bergström **) 1 Department of Medicine and the American National Red Cross Research Laboratory, New York University Medical Center, New York, New York 10016 , Ann O. Tse ***) 1 Department of Medicine and the American National Red Cross Research Laboratory, New York University Medical Center, New York, New York 10016 , A. J Johnson 1 Department of Medicine and the American National Red Cross Research Laboratory, New York University Medical Center, New York, New York 10016 › Institutsangaben Artikel empfehlen Abstract als PDF herunterladen Artikel einzeln kaufen Summary A method is described for laboratory, pilot or large-scale production of intermediatepurity human plasminogen soluble at neutral pH, with a specific proteolytic activity of approximately 12.5 CTA units/mg protein. Batches of 100-200 g can be readily produced from Fraction III paste from outdated or fresh-frozen plasma. Since this plasminogen is stable on long-term storage, and low in spontaneous plasmin activity, it is especially useful as an assay reagent (substrate) and as starting material for the preparation of high-purity plasminogen. PDF (1482 kb) Referenzen References 1 Remmert L. F, Cohen P. P. Partial purification and properties of a proteolytic enzyme of human serum. J. biol. Chem 181: 431 1949; 2 Kline D. L. The purification and crystallization of plasminogen (profibrinolysin). J. biol. Chem 204: 949 1953; 3 Sgouris J. T, Inman J. K, McCall K. B, Hyndman L. A, Anderson H. D. The preparation of humanfibrinolysin (plasmin). Vox Sang 05: 357 1960; 4 Hagan J. J, Ablondi F. B, DeRenzo E. C. Purification and biochemical properties of human plasminogen. J. biol. Chem 235: 1005 1960; 5 Kline D. L, Fishman J. B. Improved procedure for the isolation of human plasminogen. J. biol. Chem 236: 3232 1961; 6 Derechin M. Purification of human plasminogen. Biochem. J 82: 241 1962; 7 Wallén P. Studies on the purification of human plasminogen. Arkiv Kemi. 19. 451 and 469 1962 8 Hink J. H, McDonald J. K. Preparation and immunochemistry of a human euglobulin profibrinolysin. Vox Sang 08: 103 1963; 9 Shamash Y, Rimon A, Megged A, Aviram I. A method for the preparation of human plasminogen on a large scale. Vox Sang 09: 191 1964; 10 Alkjaersig N. The purification and properties of human plasminogen. Biochem. J 93: 171 1964; 11 Robbins K. C, Summaria L, Elwyn D, Barlow G. H. Further studies on the purification and characterization of human plasminogen and plasmin. J. biol. Chem 240: 541 1965; 12 Chan J. Y. S, Mertz E. T. Studies on plasminogen. V. Purification of bovine and human plasminogen by Sephadex chromatography. Canad. J. Biochem 44: 475 1966; 13 Berg W, Korsan-Bengsten K, Ygge J. Preparation of human plasminogen suitable as substrate for quantitative determination of plasminogen activators. Thrombos. Diathes. haemorrh. (Stuttg) 11: 511 1966; 14 Oncley J. L, Melin M, Richert D. A, Cameron J. W, Gross P. M. The separation of the antibodies, isoagglutinins, prothrombin, plasminogen and ß-lipoprotein into subfractions of human plasma. J. Amer. chem. Soc 71: 541 1949; 15 Alkjaersig N, Fletcher A. P, Sherry S. Epsilon-aminocaproic acid, an inhibitor of plasminogen activation. J. biol. Chem 234: 832 1959; 16 Wallén P, Bergström K. Effect of lysine on the purification of human plasminogen on cellulose ion exchangers. Acta chem. scand 13: 1464 1959; 17 Ablondi F. B, Hagan J. J, Philips M, DeRenzo E. C. Inhibition of plasmin, trypsin, and the streptokinase-activated fibrinolytic system by epsilon-aminocaproic acid. Arch. Biochem 82: 153 1959; 18 Cohn E. J, Strong L. E, Hughes W. L, Mulford D. J, Ashworth J. N, Melin M, Taylor H. L. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids. J. Amer. chem. Soc 68: 459 1946; 19 Johnson A. J, Kline D. L, Alkjaersig N. Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems. Thrombos. Diathes. haemorrh. (Stuttg) 21: 259 1969; 20 Johnson A. J, McCarty W. R, Tillett W. S, Tse A. O, Skoza L, Newman J, Semar M. Fibrinolytic, caseinolytic and biochemical methods for the study of thrombolysis in man: application and standardization. In: Blood Coagulation, Hemorrhage and Thrombosis. Tocantins L. M, Kazal L. A. Editors, 449-503 Grune and Stratton; New York: 1964 21 Bergström K, Wallén P. Removal of contaminating plasminogen from purified bovine fibrinogen. Arkiv Kemi 17: 503 1961; 22 Johnson A. J, Newman J, Howell M. B, Puszkin S. Purification of antihemophilic factor (AHF) for clinical and experimental use. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 26: 377 1967; 23 Bruhn H. D. Eine Methode zur quantitativen Bestimmung von Plasminogenaktivatoren. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 32: 27 1969; 24 Johnson A. J, Bergström K, Tse A. O. A simple procedure for the preparation of highly purified, soluble human plasminogen. (Abstract) Fed. Proc 26 (02) 647 1967;
