Determination of Fibrinogen (and Fibrinolysis) in Small Quantities of Plasma

Summary

The paper describes a simple method for the determination of fibrinogen and fibrinolysis especially suitable for serial examinations which may be performed with small amounts of plasma. In the procedures the amount of fibrin bound to the filter paper soaked in a thrombin solution is determined in the case of fibrinogen after washing, in that of fibrinolysis after incubation at 37° C and washing, by means of staining with fuchsine.

• References

• 1 Lózsa A. Papirelektroforesis készülék és methodika nagy számu szérumminta fehérje-fractióinak szeparálására és festésére. Kisérl. Orvostud 13: 86-97 1961;
• 2 Burdon K. L, McGoverns J. P, Barkin G. D, Meyers W. M. Fibrinolysis and anaphylaxis. I. Activation of blood fibrinolysin during acute anaphylactic shock in guinea pigs. J. Allergy 52: 55-62 1961;
• 3 Horányi M. Ein neues Verfahren zur Hemmung der Blutgerinnung bei der Gravimetrischen Bestimmung desnativen Fibrinogens. Klin. Wschr 20: 1128-1129 1941;
• 4 Gerendás M. Véralvadás és vérzéscsillapitás. Medicina; Budapest: 1960
• 5 Reiner M, Cheung H. L. A practical method for the determination of fibrinogen. Clin. Chem 5: 414-420 1959;
• 6 Ratnoff O. D, Menzie C. A new method for the determination of fibrinogen in small samples of plasma. J. Lab. clin. Med 37: 316-320 1951;
• 7 Parfentjev I. A, Johson M. L, Cliffton E. E. The determination of plasma fibrinogen by turbidity with ammonium sulfate. Arch. Biochem 46: 470-480 1953;
• 8 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta haemat. (Basel) 17: 237-246 1957;
• 9 Schneider Ch. L. Rapid estimation of plasma fibrinogen concentration and its use as a guide to therapy of intravascular defibrination. Amer. J. Obstet. Gynec 64: 141-147 1952;
• 10 Dóczy Á, Gerendás M, Hollcm Zs. A vèr fibrinogen tartalmának gyors meghatározása. Haemat. hung 2: 101-106 1962;
• 11 Horányi M. Fibrinopenia és sulyos vérzékenység csontvelö carcinosisban. Orv. Hetil 92: 1077-1078 1951;