The Preparation of an Artificial Reagent for the One-Stage Factor VIII Assay

D Nyman

doi:10.1055/s-0038-1654144

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1970; 23(02): 306-312
DOI: 10.1055/s-0038-1654144

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

The Preparation of an Artificial Reagent for the One-Stage Factor VIII Assay

D Nyman

¹Blood Coagulation and Fibrinolysis Laboratory (Head: Dr. F. Duckert) of the University Department of Medicine (Head: Prof .Dr. F. Koller), Bürgerspital Basel, Switzerland

› Author Affiliations

Abstract

Summary

A method for preparation of an artificial substrate for the one-stage factor VIII assay is described. Standardization of the test is given. The method is specific and reproducible.

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References

References
1 Bachmann F, Duckert F, Koller F. The Stuart-Prower Factor Assay and its Clinical Significance. Thrombos. Diathes. haemorrh. (Stuttg.) 2: 24 1958;
2 Baumgarten W, Sanders B. E, Belkin B. D, Pagenkemper F. E, Alkers W. G, Gimi-nera J. L. Antihemophilic Factor : Its Presence in Fibrinogen, Assay Problems and the Stability of Lyophilized AHF-Fibrinogen Preparations. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 354 1963;
3 Bell W. N, Alton H. G. A Brain Extract as a Substitute for Platelets Suspensions in the Thromboplastin Generation Test. Nature (Lond.) 174: 880 1954;
4 Blombäck B, Blombäck M. Purification of human and bovine fibrinogen. Ark. Kemi 10: 415 1956;
5 Bounameaux Y. Dosage du facteur VIII en un temps. Acta haemat. (Basel) 17: 355 1957;
6 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta haemat. (Basel) 17: 237 1957;
7 Das K. G, Aikat B. K. A Method for the Assay of Factor VIII with Haemophilic Plasma. Ind. J. med. Res 54: 1138 1966;
8 Davidson E, Tomlin S. ‘Thrombocytopenic Serum’ : An artificial factor VIII-deficient reagent. J. elm. Path 17: 188 1964;
9 Didisheim P. An artificial reagent for the diagnosis of classical haemophilia. Science 129: 389 1959;
10 Hardisty R. M, Macpherson J. G. A one-stage factor VIII (antihemophilic globuline) assay and its use on venous and capillary plasma. Thrombos. Diathes. haemorrh. (Stuttg.) 7: 215 1962;
11 Hjort P, Rapaport S. I, Owren P. A. A Simple, Specific One-stage Prothrombin Assay Using Russell’s Viper Venom in Cephalin Suspension. J. Lab. clin. Med 46: 89 1955;
12 Ingram G. I. G. Blood Coagulation, Factor VIII: Genetics, Physiological Control, Bioassay. Advanc. clin. Chem 8: 222 1965;
13 Jung E. G. A Rapid Quantitative Assay of Factor VIII (AHG) without the Use of Haemophilia A Plasma. Thrombos. Diathes. haemorrh. (Stuttg.) 4: 323 1960;
14 Kappeler R. Das Verhalten von Faktor V im Serum unter normalen und pathologischen Bedingungen. Klin. Med 153: 103-113 1955;
15 Koller F, Loeliger A, Duckert F. Experiments on a new clotting factor (Factor VII). Acta haemat. (Basel) 6: 1 1951;
16 Margolis J. The kaolin clotting time. J. clin. Path 11: 406 1958;
17 Milstone J. H. The Problem of the Lipid Thromboplastin. Yale J. Biol. Med 22: 675 1950;
18 Nossei H. L. The Contact Phase of Blood Coagulation. Blackwell Scientific Publications; Oxford: 1964
19 Pool J. G, Robinson J. Assay of Plasma Antihaemophilic Globulin (AHG). Brit. J. Haemat 5: 17 1959;
20 Pudlak P, Deimlova E. Quantitative Assay of Factor VIII (AHG) Using the Thrombin Generation Test. Brit. J. Haemat 12: 409 1966;
21 Schnitger H. Entwicklung eines neuartigen Gerätes zur automatischen Registrierung von Blutgerinnungszeiten und kritischer Beitrag zur Theorie anderer Meßprinzipien für gerinnungsphysiologische Untersuchungen. Thesis. Marburg (1956).
22 Stapp W. F. The Use of DEAE Cellulose in the Preparation of a Factor VIII (AHG)-free Bovine Fibrinogen Reagent. Thrombos. Diathes. haemorrh. (Stuttg.) 7: 287 1961;
23 Stormoken H. Reactivity of stored plasma to thrombin, with reference to the fibrinogen conversion accelerator and heparinoid activity. Brit. J. Haemat 3: 299 1957;
24 Triantaphyllopoulos D. G, Quick A. J, Greenwalt T. J. Action of disodiumethylenediamine-tetraacetate on blood coagulation. Evidence of the development of heparinoid activity during incubation or aeration of plasma. Blood 10: 534 1955;
25 Veder H. A. Further Purification of the Antihaemophilic Factor (AHF). Thrombos. Diathes. haemorrh. (Stuttg.) 16: 738 1966;
26 Veltkamp J. J, Orion E. F, Loeliger E. A. Detection of the Carrier State in Hereditary Coagulation Disorders. Thrombos. Diathes. haemorrh. (Stuttg.) 19: 279 1968;
27 Vinazzer H. Eine einfache quantitative Methode zur Bestimmung des Faktor VIII. Thrombos. Diathes. haemorrh. (Stuttg.) 18: 386 1967;
28 Waaler B. A. Contact activation in the intrinsic blood clotting system. Med. Diss. University Press; Oslo: 1959
29 Wolf P. Assay of Antihaemophilic Factor using the Prothrombin Conversion Ratio. Brit. J. Haemat 2: 386 1956;