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Thromb Haemost 1995; 73(05): 868-872
DOI: 10.1055/s-0038-1653883
Original Articles
Vessel Wall
Schattauer GmbH Stuttgart

Vessel Wall Injury and Arterial Thrombosis Induced by a Photochemical Reaction

Abby R Saniabadi
2   Terumo Corp,, R & D Centre, Kanagawa, Japan
,
Kazuo Umemura
1   The Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Japan
,
Nobuteru Matsumoto
2   Terumo Corp,, R & D Centre, Kanagawa, Japan
,
Sadayuki Sakuma
2   Terumo Corp,, R & D Centre, Kanagawa, Japan
,
Mitsuyoshi Nakashima
1   The Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Japan
› Author Affiliations
Further Information

Publication History

Received07 November 1994

Accepted after resubmission 19 January 1995

Publication Date:
09 July 2018 (online)

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Summary

Arterial thrombosis may be initiated in an experimental animal by a photochemical reaction between transmural green light and i.v. administered Rose Bengal, a photosensitizer dye. In this study, scanning electron microscopy has been used to reveal the nature of vessel injury and the cellular composition of the photochemically induced thrombus. A 5 mm segment of the guinea pig femoral artery was occluded by a thrombus about 10 min after irradiation with green light in the presence of systemically administered Rose Bengal. Electron microscopy revealed that following photochemical reaction, endothelial cells first contract and, with further irradiation, become detached from the vessel wall, with their cell membrane being destroyed at the irradiated site where an occlusive platelet-rich thrombus was formed. Endothelial cell injury and vessel occlusion could be completely inhibited by the aminothiol, DL-cysteine administered i.v. 1 min after Rose Bengal. The mechanism of endothelial injury in this model appears to be by singlet molecular oxygen, 1O2 formed by energy transfer from the photo-excited dye to O2.

 

  • References

  • 1 Meade TW. The epidemiology of haemostatic and other variables in coronary artery disease. In: Thrombosis and Haemostasis. Verstraete M, Ver-mylen J, Lijnen R, Amout J. eds Leuven University Press; Leuven: 1987. pp 37-60
    Google Scholar
  • 2 Warlow C. Antithrombotic drugs and the prevention of stroke. In: Thrombosis and Haemostasis. Verstraete M, Vermylen J, Lijnen R, Amout J. eds Leuven University Press; Leuven: 1987. pp 301-324
    Google Scholar
  • 3 Moncada S, Vane JR. Arachidonic acid metabolites and the interactions between platelets and blood-vessel walls. N Engl J Med 1979; 300: 1142-1147
    CrossrefPubMedGoogle Scholar
  • 4 Nakashima M. A Novel Photochemical Model for Thrombosis Research and Evaluation of Antithrombotic and Thrombolytic Agents. Churchill Livingstone; New York: 1994
    Google Scholar
  • 5 Romson JL, Haack DW, Lucchesi BR. Electrical induction of coronary artery thrombosis in the ambulatory canine: a model for in vivo evaluation of antithrombotic agents. Thromb Res 1980; 17: 8441-8453
    PubMedGoogle Scholar
  • 6 Grand L, Becker FF. Mechanism of inflammation, I: laser-induced thrombosis, a morphologic analysis. Proc Soc Exp Biol 1965; 119: 1123-1129
    CrossrefPubMedGoogle Scholar
  • 7 Moore S, Friedman RJ, Singal DP, Gauldie J, Blajchman MA. Inhibition of injury induced thromboatherosclerotic lesions by anti-platelet serum in rabbits. Thromb Res 1976; 35: 70-79
    PubMedGoogle Scholar
  • 8 Steele PM, Chesebro JH, Stanson AW, Holmes DR, Dewanjee MK. Balloon angioplasty: natural history of pathophysiological response to injury in a pig model. Circ Res 1985; 57: 105-112
    CrossrefPubMedGoogle Scholar
  • 9 Kurz KD, Main BW, Sandusky GE. Rat model of arterial thrombosis induced by ferric chloride. Thromb Res 1990; 60: 269-280
    CrossrefPubMedGoogle Scholar
  • 10 Watson BD, Dietrich WD, Busto R, Wachtel NS, Ginsberg MD. Induction of reproducible brain infarction by photochemically initiated thrombosis. Ann Neurol 1985; 17: 497-504
    CrossrefPubMedGoogle Scholar
  • 11 Saniabadi AR. Photosensitisers and photochemical reactions. In: A Novel Photochemical Model for Thrombosis Research and Evaluation of Antithrombotic and Thrombolytic Agents. Nakashima M. ed Churchill Livingstone; New York: 1994. pp 1-19
    Google Scholar
  • 12 Vandeplassche G, Bernier M, Kusama BM, Hearse DJ. Singlet oxygen and myocardial injury: ultrastructural, cytochemical and electrocardiographic consequences of photoactivation of rose bengal. J Mol Cell Cardiol 1990; 22: 287-301
    CrossrefPubMedGoogle Scholar
  • 13 Hearse DJ, Kusama Y, Bernier M. Rapid electrophysiological changes leading to arrhythmias in the aerobic rat heart. Photosensitization studies with rose bengal-derived reactive oxygen intermediates Circ Res 1989; 65: 146-153
    CrossrefPubMedGoogle Scholar
  • 14 Ito T. Photodynamic agents as tools in cell biology. Photochem Photobiol Rev 1983; 7: 141-186
    PubMedGoogle Scholar
  • 15 Singh H, Vadasz JA. coli ribosomes. Photochem Photobiol 1978; 28: 539-545
    CrossrefPubMedGoogle Scholar
  • 16 Hall RD, Chignell CF. Steady-state near-infrared detection of singlet molecular oxygen: a quenching experiment with sodium azide. Photochem Photobiol 1987; 45: 459-464
    CrossrefPubMedGoogle Scholar
  • 17 Anson SW, Li H, Roethling P, Cummings KB, Chignell CF. O2 -photogenerated from aqueous solutions of tetracycline antibiotics (pH 7.3) as evidenced by DMPO spin trapping and cytochrome c reduction. Biochem Biophys Res Commun 1987; 146: 1191-1195
    CrossrefPubMedGoogle Scholar
  • 18 Arnason JT, Guerin B, Kraml MM, Mehta B, Redmond RW, Scaiano JC. Phototoxic and photochemical properties of sanguinarine. Photochem Photobiol 1992; 55: 35-38
    CrossrefPubMedGoogle Scholar
  • 19 Anderson R, Eftychis HA, Weiner A, Findlay GH. An in vivo and in vitro investigation of the phototoxic potential of tenoxicam, a new non-steroidal anti-inflammatory agent. Dermatologica 1987; 175: 229-234
    CrossrefPubMedGoogle Scholar
  • 20 Halliwell B, Gutteridge JM C. The chemistry of oxygen radicals and other oxygen-derived species. In: Free Radicals in Biology and Medicine. Halliwell B, Gutteridge J MC. eds Clarendon Press, Oxford; 1989: pp22-276
    Google Scholar
  • 21 Knofsky JR, Hoogland H, Wever R, Weiss SJ. Singlet oxygen production by human eosinophils. J Biol Chem 1988; 263: 9692-9696
    PubMedGoogle Scholar
  • 22 Harlan J. Leucocyte-endothelial interactions. Blood 1985; 65: 513-525
    PubMedGoogle Scholar
  • 23 Matheson IB, Kratowich NR, Lee J. The quenching of singlet oxygen by amino acids and proteins. Photochem Photobiol 1975; 21: 165-171
    CrossrefPubMedGoogle Scholar
  • 24 Bowman WC, Rand MJ. Constituents of tissues. In: Textbook of Pharmacology. Bowman WC, Rand MJ. eds Blackwell, Oxford; 1980: 1.1-1.50
    Google Scholar
  • 25 Malkinson ID, Hanson WR. Radiobiology of the skin. In: Physiology, Biochemistry and Molecular Biology of the Skin. Goldsmith LA. ed Oxford University Press, Oxford; 1991. pp 976-1016
    Google Scholar
  • 26 Takiguchi Y, Wada K, Mizuno A. Evaluation of antithrombotic drugs. In: A Novel Photochemical Model for Thrombosis Research and Evaluation of Antithrombotic and Thrombolytic Agents. Nakashima M. ed Churchill Livingstone; New York: 1994. pp 39-53
    Google Scholar
  • 27 Nakashima M. Enhancement of thrombolytic efficacy of tissue-type plasminogen activator by adjuvants in the guinea pig thrombosis model. Eur J Pharmacol 1994; 264: 191-198
    CrossrefPubMedGoogle Scholar
  • 28 Nakashima M, Nishiyama H. Thrombolysis and thrombolytic drugs: arterial intimai thickening after thrombolysis. In: A Novel Photochemical Model for Thrombosis Research and Evaluation of Antithrombotic and Thrombolytic Agents. Nakashima M. ed Churchill Livingstone; New York: 1994. pp 67-84
    Google Scholar