Summary
A method for preparation of fibrinogen from human plasma is presented. The method,
a modification of the glycine precipitation procedure of Kazal (6), is simple and
rapid, requiring 8 hours or less. The fibrinogen solution is stable on storage in
the refrigerator for at least 7 months, represents an average yield of 30-40% of that
in the starting material, and is around 95% clottable. Clottability is good even on
storage at 37° C for several days; it is generally around 80% clottable after this
storage period. The solution is free of plasminogen and plasmin but contains trace
amounts of FSF (fibrin stabilizing factor) and factor VIII (factor VIII activity tends to disappear with storage). Ultracentrifugal analysis shows a main
peak comprising approximately 95% of the total area (apparent sedimentation coefficient
5.8 × 10-13) and a smaller peak comprising approximately 5% or less of the total area (apparent
sedimentation coefficient 3-3.5 × 10−13).
