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Thromb Haemost 1971; 26(01): 077-082
DOI: 10.1055/s-0038-1653657
Originalarbeiten – Original Articles – Travaux Originaux
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Fibrinolysis Induced by Factor XII Contained in Prothrombin Preparations

R. H Landaburu
1   Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina
,
C Pernici
1   Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina
,
D. E Castellanos
1   Institute of Pharmacology, National University of Cordoba, Cordoba, Argentina
› Author Affiliations
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Publication History

Publication Date:
28 June 2018 (online)

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Summary

Prothrombin products prepared by Seegers procedure induce fibrinolysis when injected intravenously. This property is due to a contaminant denominated Prothrombin Activating Derivative-B, identified as factor XII. The fibrinolytic fraction accompanies prothrombin throughout the purification procedure and may be separated by chromatography on DEAE cellulose.

 

  • References

  • 1 Celander D. R, Guest M. M. Euglobulin lysis time. In: Blood coagulation, hemorrhage and thrombosis. 249-250 2nd. ed. Edited by Tocantins L. M, Kazal L. A. Grune & Stratton, Inc; New York: 1964
    Google Scholar
  • 2 Guest M. M. Estimation of fibrinolytic activity of plasma or serum: Fibrin plate method, unheated and heated (Methods of Astrup and Mullertz and Lassen). In: Blood coagulation, hemorrhage and thrombosis. 246-248 2nd. ed. Edited by Tocantins L. M, Kazal L. A. Grune & Stratton, Inc; New York: 1964
    Google Scholar
  • 3 Hunter D. T, Allensworth J. Quantitative assay of the Hageman factor. Amer. J. clin. Path 47: 448 1967;
    PubMedGoogle Scholar
  • 4 Iatridis S. G, Ferguson J. H. Effect of surface and Hageman factor on the endogenous or spontaneous activation of the fibrinolytic system. Thrombos. Diathes. haemorrh. (Stuttg.) 06: 411 1961;
    PubMedGoogle Scholar
  • 5 Kowalski E, Budzyński A. Z, Kopeć M, Latallo Z. S, Lipiński B, Wegrzynowicz Z. Circulating fibrinogen degradation products (FDP) in dog blood after intravenous thrombin in fusion. Thrombos. Diathes. haemorrh. (Stuttg.) 13: 12 1965;
    PubMedGoogle Scholar
  • 6 Landaburu B. H, Albado E. Factor X precursor in purified bovine prothrombin preparations. Coagulations 02: 269 1969;
    PubMedGoogle Scholar
  • 7 Landaburu B. H, Albado E. Activation of factor X in bovine prothrombin preparations. Thrombos. Diathes. haemorrh. (Stuttg.) 24: 338 1970;
    PubMedGoogle Scholar
  • 8 Landaburu B. H, Abdala O, Morrone M. J. Proteolytic enzymes derived from prothrombin. Canad. J. Biochem 43: 417 1965;
    CrossrefPubMedGoogle Scholar
  • 9 Landaburu B. H, Gurtino J. A standard preparation of bovine fibrinogen. Thrombos. Diathes. haemorrh. (Stuttg.) 12: 64 1964;
    PubMedGoogle Scholar
  • 10 Landaburu B. H, Giavedoni E, Albado E. Factor XII in boyine prothrombin preparations: A purification procedure. Coagulation. (In press.)
    PubMedGoogle Scholar
  • 11 Landaburu B. H, Giavedoni E, Santillân B. Thrombin and acetylated thrombin in the activation of fibrinolysis. Canad. J. Physiol. Pharmacol 46: 809 1968;
    CrossrefPubMedGoogle Scholar
  • 12 Landaburu B. H, Lima F, Albado E. Activation characteristics of bovine prothrombin preparations. Brit. J. Haemat 17: 83 1969;
    CrossrefPubMedGoogle Scholar
  • 13 Landaburu B. H, Seegers W. H. The acétylation of thrombin. Canad. J. Biochem 37: 1361 1959;
    PubMedGoogle Scholar
  • 14 Moser K. M, Frey MBelle. Comparison of caseinolytic and fibrinolytic assays for plasmin (fibrinolysin) in fibrinolytic agents. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 252 1966;
    PubMedGoogle Scholar
  • 15 Ogston D, Ogston C. M, Batnoff O. D, Forbes C. D. Studies on a complex mechanism for the activation of plasminogen by kaolin and by chloroform : the participation of Hageman factor and additional cofactors. J. clin. Invest 48: 1786 1969;
    CrossrefPubMedGoogle Scholar
  • 16 Batnoff O. D, Menzie C. New method for determination of fibrinogen in small samples of plasma. J. Lab. clin. Med 37: 316 1951;
    PubMedGoogle Scholar
  • 17 Seegers W. H. The purification of prothrombin. Record. Chem. Progr. (Kreske-Hocker Sci. Lib.) 13: 143 1952;
    PubMedGoogle Scholar
  • 18 Seegers W. H, Landaburu B. H, Johnson J. F. Thrombin-E as a fibrinolytic enzyme. Science 131: 726 1960;
    CrossrefPubMedGoogle Scholar
  • 19 Seegers W. H, Levine W. G, Shepard B. S. Further studies on the purification of thrombin. Canad. J. Biochem 36: 603 1958;
    PubMedGoogle Scholar
  • 20 Seegers W. H, Smith H. P. Factors which influence the activity of purified thrombin. Amer. J. Physiol 137: 348 1942;
    PubMedGoogle Scholar
  • 21 Ware A. G, Seegers W. H. Two-stage procedure for the quantitative determination of prothrombin concentration. Amer. J. clin. Pathol 19: 471 1949;
    PubMedGoogle Scholar