Platelet Changes Induced by Elatericin A

Summary

Elatericin A at a concentration of 3 μg/ml causes spicule formation on platelets and inhibits spreading on glass surfaces, viscous metamorphosis and clot retraction. Elatericin A has no effect whatsoever on the activity of isolated platelet factors. However platelet factor 3-availability is decreased by elatericin A. Platelets treated with elatericin A up to a limiting concentration of 30 μg/ml regain their clot retracting activity if washed and reincubated in normal plasma.

Elatericin A does not affect the ATP content nor the ATPase activity of platelets. A normal ADP-release is obtained upon addition of thrombin to elatericin A treated platelets. Addition of ATP and Mg ions does not restore the clot retracting activity of elatericin A treated platelets.

• References

• 1 Shohat B. The influence of cucurbitacins on tumor cells. Ph. D. Thesis. The Hebrew University, Jerusalem; Israel: 1965
• 2 Gitter S, Gallily J. R, Shohat B, Lavie D. Studies on the anti tumor properties of cucurbitacins. Cancer Res 21: 516 1961;
• 3 Gallily R, Shohat B, Gitter S, Kalish Y, Lavie D. Further studies on the antitumor properties of cucurbitacins. Cancer Res 22: 1038 1962;
• 4 Shohat B, Gitter S, Lavie D. Antitumor activity of cucurbitacins: metabolic aspects. Cancer Chem. Rep 23: 1923 1962;
• 5 Shohat B, Gitter S, Lavie D. The action of elatericin A on human leukemic and normal lymphocytes. J. nat. Cancer Inst 38: 1 1967;
• 6 Biggs M, Macfarlane R. G. Human Blood coagulation and its disorders. Third Edit.. Blackwell Scientific Publications; Oxford: 1962
• 7 Käser-Glanzmann R, Lüscher E. F. The mechanism of platelet aggregation in relation to hemostasis. Thrombos. Diathes. haemorrh. (Stuttg.) 07: 480 1962;
• 8 Waller H. D, Lohr G. W, Gringnani F, Gross R. Über den Energiestoffwechsel normaler menschlicher Thrombozyten. Thrombos. Diathes. haemorrh. (Stuttg.) 03: 520 1959;
• 9 Zaizov R, Cohen I, Matoth Y. Thrombasthenia: A Study of Two Siblings. Acta paed, scand.. 1968 (In press)
• 10 Fantl P, Ebbels L, Nelson J. E. Brit. J. exp. Path 32: 538 1951;
• 11 Hartert H. Blutgerinnungsstudien mit der Thrombelastographie, einem neuen Untersuchungsverfahren. Klin. Wschr. 1948: 577.
• 12 Stefanini M, Dameshek W. The hemorrhagic disorders. 15 Grüne and Stratton; New York: 1955
• 13 Jürgens J, Beller F. K. In: Klinische Methoden der Blutgerinnungsanalyse. 145 Stuttgart: 1959
• 14 Spaet T. H, Cintron J. Studies on platelet factor-3 availability. Brit. J. Haemat 11: 269 1965;
• 15 Hardisty R. M, Dormondy K. M, Hutton R. A. Thrombasthenia: studies on three cases. Brit. J. Haemat 10: 371 1964;
• 16 Adam M. In: Methods of Enzymatic Analysis. 539 Bergmeyer H. U. edit. Academic Press; New York and London: 1963
• 17 Bettex-Galland M, Lüscher E. F. Extraction of an actomyosin like protein from human thrombocytes. Nature (Lond.) 184: 276 1959;
• 18 Bettex-Galland M, Lüscher E. F. Studies on the metabolism of blood platelets in relation to clot retraction. Thrombos. Diathes. haemorrh 04: 178 1960;
• 19 Gross R, Lohr G. W, Waller H. D. Proc. IV Int. Congr. Biochemistry, Vienne. 1958. Pergamon Press; London:
• 20 Breddin K, Longbein H. Über den Einfluß verschiedener Stoffwechselhemmer auf die Thrombozytenfunktion. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 29 1963;
• 21 Bounameaux Y. Sur le mécanisme de la rétraction du caillot et de la métamorphose visqueuse des plaquettes. Rev. Hémat 12: 16 1957;
• 22 Bettex-Galland M, Lüscher E. F. The Contractile Protein from Blood Platelets. Advanc. Prot. Chem 20: 1 1965;
• 23 Zbinden G, Gamble Jr J. L, Jackson D. P. Effects of thrombin on the potassium and ATP content of platelets. J. clin. Invest 43: 2063 1964;
• 24 Harrington W. J. General discussion on Metabolism of Platelets. In: The blood platelets. 433 Henry Ford Hospital International Symposium Boston; 1961