Download PDF
Thromb Haemost 1969; 21(01): 129-133
DOI: 10.1055/s-0038-1653517
Originalarbeiten - Original Articles - Travaux Originaux
Schattauer GmbH

In Vivo and In Vitro Binding of Factor VIII to Human Platelets[*]

Margaret Howell Karpatkin
1   Department of Medicine, New York University Medical School and the American National Red Cross Research Laboratory, New York, N. Y. 10016
,
S Karpatkin
1   Department of Medicine, New York University Medical School and the American National Red Cross Research Laboratory, New York, N. Y. 10016
› Author Affiliations
Further Information

Publication History

Publication Date:
10 June 2018 (online)

    Permissions and Reprints

Summary

Human platelets were tested for the presence of factor VIII employing a modified partial thromboplastin system. Factor VIII was present on the surface of normal platelets after they had been washed five times in human Ringer solution containing 0.1 mM EDTA. Washed platelets from VIII deficient patients adsorbed factor VIII from normal plasma both in vitro and in vivo.

* Supported in part by a grant-in-aid from the New York Heart Association.


 

  • References

  • 1 Schijfman S, Rapaport S. I, Chong M. M. Y. The mandatory role of lipid in the interaction of factors VIII and IX. Proc. Soc. exp. Biol. (N.Y) 123: 736 1966;
    CrossrefPubMedGoogle Scholar
  • 2 Lundblad R. L, Davie E. W. The activation of antihemophilic factor (factor VIII) by activated Christmas factor (activated factor IX). Biochem 03: 1720 1964;
    CrossrefPubMedGoogle Scholar
  • 3 Macfarlane R. G. The coagulant action of Russell’s viper venom: the use of antivenom in defining its reaction with a serum factor. Brit. J. Haemat 07: 496 1961;
    CrossrefPubMedGoogle Scholar
  • 4 Zucker-Franklin D, Spaet T. H. Partial purification of intermediate coagulation product I and a study of its properties. Amer. J. Physiol 205: 341 1963;
    PubMedGoogle Scholar
  • 5 Lundblad R. L, Davie E. W. The activation of Stuart factor (factor X) by activated antihemophilic factor (activated factor VIII). Biochem 04: 113 1965;
    CrossrefPubMedGoogle Scholar
  • 6 Jobin F, Esnouf M. P. Studies on the formation of the prothrombin-converting complex. Biochem. J 102: 666 1967;
    CrossrefPubMedGoogle Scholar
  • 7 Marcus A. J, Zucker M. B. The physiology of blood platelets. 22 Grune and Stratton; New York: 1965
    Google Scholar
  • 8 Hjort P, Rapaport S. I, Owren P. A. Evidence that platelet accelerator (platelet factor I) is adsorbed plasma proaccelerin. Blood 10: 1139 1955;
    PubMedGoogle Scholar
  • 9 Seibert R. H, Margolius A, Ratnoff O. D. Observations on hemophilia, parahemophilia, and coexistent hemophilia and parahemophilia. J. Lab. clin. Med 52: 449 1958;
    PubMedGoogle Scholar
  • 10 Horowitz H. I, Fujimoto M. M. Association of factors XI and XII with blood platelets. Proc. Soc. exp. Biol. (N. Y) 119: 487 1965;
    CrossrefPubMedGoogle Scholar
  • 11 Ware A. G, Fahey J. L, Seegers W. H. Platelet extracts, fibrin formation and interaction of purified prothrombin and thromboplastin. Amer. J. Physiol 154: 140 1948;
    PubMedGoogle Scholar
  • 12 Raccuglia G. Factor VIII (antihemophilic factor) and factor V (proaccelerin) activity of platelets. Proc. Soc. exp. Biol. (N. Y) 104: 309 1960;
    CrossrefPubMedGoogle Scholar
  • 13 Nachman R. L, Marcus A. J, Zucker-Franklin D. Subcellular localisation of platelet fibrinogen. Blood 24: 853 1964;
    PubMedGoogle Scholar
  • 14 Bounameaux Y. Dosage des facteurs de coagulation contenus dans l’atmosphère plasmatique des plaquettes humaines. Rev. franç. Étud. clin. biol 02: 52 1957;
    PubMedGoogle Scholar
  • 15 Lüscher E. F. Ein fibrinstabilisierender Faktor aus Thrombozyten. Schweiz, med. Wschr 87: 1220 1957;
    PubMedGoogle Scholar
  • 16 Lüscher E. F. Thrombocyten-Faktoren. Ergebn. Physiol 50: 1 1959;
    CrossrefPubMedGoogle Scholar
  • 17 Minot G. R, Lee R. I. The blood platelets in hemophilia. Arch, intern. Med 25: 474 1916;
    PubMedGoogle Scholar
  • 18 Patek A. J, Stetson R. P. Hemophilia I. The abnormal coagulation of the blood and its relation to the blood platelets. J. clin. Invest 15: 531 1936;
    PubMedGoogle Scholar
  • 19 Mann F. D, Hum M, Magah T. B. Observations on the conversion of prothrombin to thrombin. Proc. Soc. exp. Biol. (N. Y) 66: 33 1947;
    CrossrefPubMedGoogle Scholar
  • 20 latridis P. G, Ferguson J. H, latridis S. G. Surface factor mechanisms in relation to blood platelets : Evidence that activated Hageman factor is present on the surface of platelets. Thrombos. Diathes. haemorrh. (Stuttg) 11: 355 1964;
    PubMedGoogle Scholar
  • 21 Borchgrevink G. F, Owren P. A. The hemostatic effect of normal platelets in hemophilia and factor V deficiency. Actamed. scand 170: 375 1961;
    PubMedGoogle Scholar
  • 22 Karpatkin S. Studies on human platelet glycolysis. Effect of glucose, cyanide, insulin, citrate and agglutination and contraction on platelet glycolysis. J. clin. Invest 46: 409 1967;
    PubMedGoogle Scholar
  • 23 Johnson A. J, Newman J, Howell M. B, Puszkin S. Two large scale procedures for purification of human antihemophilic factor (AHF). Blood 28: 1011 1966;
    PubMedGoogle Scholar
  • 24 Hemker H. C, Kahn M. J. P. Reaction sequence of blood coagulation. Nature (Lond) 215: 1201 1967;
    CrossrefPubMedGoogle Scholar