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Thromb Haemost 1981; 46(02): 543-546
DOI: 10.1055/s-0038-1653406
Original Article
Schattauer GmbH Stuttgart

Production of Cryoprecipitate of Intermediate Purity in a Closed System Thaw-Siphon Process

Ernest C Mason
*   The Red Cross Blood Transfusion Service, Brisbane, Queensland, Australia
,
Duncan S Pepper
**   The Scottish National Blood Transfusion Service, Headquarters Unit Laboratory, Edinburgh, Scotland
,
Brenda Griffin
**   The Scottish National Blood Transfusion Service, Headquarters Unit Laboratory, Edinburgh, Scotland
› Author Affiliations
Further Information

Publication History

Received 10 April 1981

Accepted 05 June 1981

Publication Date:
05 July 2018 (online)

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Summary

The thaw-siphon procedure for routine production of cryoprecipitate is described briefly, and also its extension to a reproducible procedure for production of cryoprecipitates of intermediate purity. For the latter, the average yield of factor VIII is 60% of that in the prefrozon plasma and approximately 30% is present in the supernatant plasma. The product contains 0.53 units of VIII:C per mg of total protein (of which about 70% is clottable). The mean value of the ratio VIII:C/VIII:RAG is about 0.7. The yield and purity of the product compare favourably with those reported for intermediate purity factor VIII concentrates produced by large-scale plasma fractionation laboratories. The procedure offers an alternative pathway to the production of factor VIII concentrate of intermediate purity and can be operated in any blood bank with a minimum of equipment.

Keywords

F VIII: C - Cryoprecipitation - Thaw-siphon
 

  • References

  • 1 Mason EC. Thaw-siphon technique for production of cryoprecipitate of factor VIII. Lancet 1978; 2: 15-17
    PubMedSearch in Google Scholar
  • 2 Mason EC, Harden PA, Shaw AE. The thaw-siphon procedure for cryoprecipitate production: an alternative pathway to an improved intermediate AHF concentrate within a closed pack system. XVth Jt Cong Intern Soc Haemat & Intern Soc Blood Transfusion, Paris 1978; Absts p. 603
    PubMedSearch in Google Scholar
  • 3 Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J Biol Chem 1949; 177: 751-766
    PubMedSearch in Google Scholar
  • 4 Ellis BC, Stransky A. A quick and accurate method for the determination of fibrinogen in plasma. J. Lab Clin Med 1961; 58: 477-488
    PubMedSearch in Google Scholar
  • 5 Mason EC. Thaw-siphon technique for factor VIII cryoprecipitate. Lancet 1978; 2: 636-637
    PubMedSearch in Google Scholar
  • 6 Doorenbos S, Das PC. Red Cross Blood Bank, Groningen, The Netherlands — Personal Communication. 1980
    PubMedSearch in Google Scholar
  • 7 Prowse CV, McGill A. Evaluation of the “Mason” (continuous-thaw-siphon) method for cryoprecipitate production. Vox Sang 1979; 37: 235-243
    PubMedSearch in Google Scholar
  • 8 Newman J, Johnson AJ, Karpatkin MH, Puszkin S. Methods for the production of clinically effective intermediate and high-purity factor VIII concentrates. Br J Haematol 1971; 21: 1-20
    CrossrefPubMedSearch in Google Scholar
  • 9 Wickerhauser M, Mercer JE, Eckenrode JW. Development of large scale fractionation methods. VI. An improved method for preparation of anti-haemophilic factor. Vox Sang 1978; 35: 18-31
    CrossrefPubMedSearch in Google Scholar
  • 10 Pool JG, Hershgold EJ, Pappenhagen AR. High-potency anti-haemophilic factor concentrate prepared from cryoglobulin precipitate. Nature 1964; 203: 312
    PubMedSearch in Google Scholar
  • 11 Pool JG, Shannon AE. Production of high-potency concentrates of anti-haemophilic globulin in a closed bag system. New Eng J Med 1965; 273: 1443-1447
    CrossrefPubMedSearch in Google Scholar
  • 12 Haff LA. Production of Ficoll, Percoll and albumin gradients by the freeze-thaw method. Prep Biochem 1979; 9: 149-156
    PubMedSearch in Google Scholar