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Thromb Haemost 1981; 46(02): 479-484
DOI: 10.1055/s-0038-1653392
Original Article
Schattauer GmbH Stuttgart

Characterization of a Form of Bovine Factor VIII Which Does Not Aggregate Human Platelets

Edward P Kirby
The Department of Biochemistry and Specialized Center of Research on Thrombosis, Temple Univ. Health Sciences Center, Philadelphia, Pa., U.S.A.
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Further Information

Publication History

Received 10 November 1980

Accepted 04 May 1981

Publication Date:
05 July 2018 (online)

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Summary

Fractionation of partially purified bovine factor VIII on tricalcium citrate columns produces a material which contains high levels of factor VIII procoagulant activity and factor VIII- related antigen, but does not aggregate human platelets. The procoagulant activity can be blocked by human inhibitors of factor VIII: C and by rabbit antibody to bovine factor VIII. Its activity can be increased by thrombin modification. The apparent molecular weight of this form of factor VIII is significantly lower than that of the forms of factor VIII which contain platelet aggregating activity, and it exhibits a higher mobility on agarose electrophoresis.

Keywords

Factor VIII - Bovine - Platelet aggregation - von Willebrand factor
 

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