Experimental DIC Developed In Dogs With Pulmonary And Renal Involvement Induced By Oleic Acid And Anti-Lung Antibody

In order to have pathogenetic comprehension on the causal relationship between shock state and development of DIC, the following experiments were carried out and compared with the clinical and laboratory findings in human cases with pulmonary and/or renal diseases. An ordinary shock state was invited in dogs by means of exsanguination, depressing mean blood pressure down to 70mmHg and sustaining it for one hour, but almost no changes were realized in coagulation and fibrinolysis parameters including platelet count and functions of blood as well as the heart rate and cardiac index of animals.

The additional injection of a rather small amount of thromboplastin, however, caused typical hematological changes of DIC and demonstrable disturbance of pulmonary-cardiac (p-c) functions which were restored spontaneously. Repeated injections of oleic acid introduced significant histological changes in the lung and kidney of dogs, but only slight shifts were proved in hematological findings and p-c functions and not so much modification of them was noticed even under the exsanguination shock state. The additional injection of thromboplastin, however, showed exaggerated histological and functional changes.

The long term injections of anti-lung antibody to dogs introduced a certain kind of histological changes, similar to those of so-called “Goodpasture’s syndrome” in the lung and kidney as well as blood findings like DIC and p-c disturbance, and the simultaneous manipulation of exsanguination and thromboplastin injection promoted those changes more gravely.

The same kind of exaggerated reactions in blood findings and p-c functions was encountered in clinical cases with lung and/or kidney disorders and suggested that these damages might play much role in the development of DIC.