Identification Of Membrane-Linked Proteins Acting On Blood Red Cell Filtration. Study In Cerebrovascular Accident And Thrombosis

The blood red cell filtration (RCF) through nucleopore filters(5 μ ø) decrease in cerebrovascular accidents (CVA) and thrombosis. This disorder is related to circulating factors, especially fibrinogen and factor VIII. But after washing it also appears to be related to membrane-1 inked proteins. In this study, isolation and identification of these proteins were realized in patients with increased RCF. Have been here concerned 20 patients with CVA and/or other thrombosis, 8 days after the onset and compared to 30 controls.

After the RC have been washed twice the proteins were eluted from the membrane, concentrated and submitted to usual electrophoresis and immunoelectrophoresis. The identification was made with specific antiserums against factor VIII, fibrinogen, fragments D and E, transferin, coeruloplasmin and using SDS polyacrylamid gel electrophoresis with, as controls, purified fibrinogen, fragments D and E, transferin, coeruloplasmin.

The results were : in controls the eluted proteins are similar with an albumin/globulin ratio (A/G ) > 1. In patients with a high disorder of RCF the proteins were increased, with an inversion of the A/G. Two kinds of proteins were found : (1) a β population composed of inflammatory proteins, mainly transferin, (2), a β-α population consistuted by fibrin-related products. Concerning the whole population of patients with a high disorder in RCF, 80 % exhibited proteins induced by inflammation. In 20 % it was found proteins related to the coagulation, i.e. : fibrin related products. Finally, the disorder of RCF in thrombosis is much more due to these membrane disturbances than to circulating factors. During the clinical care of the patients it also has been observed that pentoxyphiline improved the RCF, certainly acting at the membrane level.