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Thromb Haemost 1981; 46(01): 120
DOI: 10.1055/s-0038-1652315
Coagulation – VII: Prothrombin, Vitamin K-dependent Factors
Schattauer GmbH Stuttgart

β-Propiolactone/Ultraviolet Irradiation: Quantitative Studies On Effectiveness For Inactivation Of Hepatitis B Virus

A M Prince
1   The Lindsley F. Kimball Research Institute of The New York Blood Center, New York, New York
3   The Liberian Institute for Biomedical Research, Robertsfield, Liberia, New York
4   Dept. of Pathology, New York Hospital-Cornell Medical Center, New York, New York
,
W Stephan
2   Biotest-Serum-Institut GmbH, Frankfurt, Germany
,
B Brotman
1   The Lindsley F. Kimball Research Institute of The New York Blood Center, New York, New York
3   The Liberian Institute for Biomedical Research, Robertsfield, Liberia, New York
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Publikationsdatum:
24. Juli 2018 (online)

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The efficacy of combined β-Propiolactone/Ultraviolet irradiation (βPL/UV) for inactivation of hepatitis B virus in labile blood derivitives has been reinvestigated. To permit quantitative estimation of process efficacy, a regression analysis of the relation between virus dose and incubation period was carried out. This has provided a means for estimating virus titer and for determining the accuracy of such estimates.

The data suggest that βPL/UV can reduce virus titer about 10,000,000 fold (10-7). The process efficacy for βPL/UV followed by the Aerosil adsorption procedure used in preparation of stabilized human serum (Biseko) containing most human serum proteins except for removal of factor VIII, the PPSB factors, fibrinogen and the lipoproteins was estimated to be about 10-8.

This degree of virus inactivation should be more than sufficient to sterilize the highest amounts of hepatitis B virus which could be expected in pooled human plasma which has been screened for HBsAg.