Download PDF
Thromb Haemost 1977; 38(04): 1002-1009
DOI: 10.1055/s-0038-1651918
Original Article
Schattauer GmbH

Malondialdehyde Production by Platelets during Secondary Aggregation

D. E Macfarlane
1   Specialized Center for Thrombosis Research, Temple University Hospital, 3401 North Broad Street, Philadelphia, Pennsylvania, 19140, U.S.A.
,
Sheila Gardner
1   Specialized Center for Thrombosis Research, Temple University Hospital, 3401 North Broad Street, Philadelphia, Pennsylvania, 19140, U.S.A.
,
Carol Lipson
1   Specialized Center for Thrombosis Research, Temple University Hospital, 3401 North Broad Street, Philadelphia, Pennsylvania, 19140, U.S.A.
,
D. C. B Mills
1   Specialized Center for Thrombosis Research, Temple University Hospital, 3401 North Broad Street, Philadelphia, Pennsylvania, 19140, U.S.A.
› Author Affiliations
Further Information

Publication History

Publication Date:
04 July 2018 (online)

    Permissions and Reprints

Summary

A method is described for increasing the sensitivity of the thiobarbiturate assay for malondialdehyde by concentrating the coloured reaction product. The basal level of malondialdehyde-like material in plasma was found to be about 0.03 μM. Platelets synthesized malondialdehyde when stimulated by collagen or thrombin and also during the second phase of aggregation induced by ADP or adrenaline.

 

  • References

  • 1 Bernheim F, Bernheim M. L. C, Wilbur K. M. 1947; The reaction between thiobarbituric acid and oxidation products of certain lipides. Journal of Biological Chemistry 174: 257.
    PubMedGoogle Scholar
  • 2 Evans G, Packham M. A, Nishizawa E. E, Mustard J. F, Murphy E. A. 1968; Effect of acetylsalicylic acid on platelet function. Journal of Experimental Medicine 128: 877.
    CrossrefPubMedGoogle Scholar
  • 3 Flower R. J. 1974; Drugs which inhibit prostaglandin biosynthesis. Pharmacological Reviews 26: 33.
    PubMedGoogle Scholar
  • 4 Hamberg M, Samuelsson B. 1973; Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis. Proceedings of the National Academy of Sciences (U.S.A.) 70: 899.
    CrossrefPubMedGoogle Scholar
  • 5 Hamberg M, Samuelsson B. 1974; Prostaglandin endoperoxides. Novel transformations of arachidonic acid in human platelets. Proceedings of the National Academy of Science 71: 3400.
    CrossrefPubMedGoogle Scholar
  • 6 Holmsen H, Østvold A.-C, Day H. J. 1973; Behaviour of endogenous and newly absorbed serotonin in the platelet release reaction. Biochemical Pharmacology 22: 2599.
    CrossrefPubMedGoogle Scholar
  • 7 Karpatkin S, Strick N. 1972; Heterogeneity of human platelets. V. Differences in glycolytic and related enzymes with possible relation to platelet age. Journal of Clinical Investigation 51: 1235.
    CrossrefPubMedGoogle Scholar
  • 8 Kocsis J. J, Hernandovich J, Silver M. J, Smith J. B, Ingerman C. M. 1973; Duration of inhibiton of platelet prostaglandin formation and aggregation by ingested aspirin or indomethacin. Prostaglandins 3: 141.
    CrossrefPubMedGoogle Scholar
  • 9 Macfarlane D. E, Mills D. C. B. 1976; Methylmercury, prostaglandin synthesis and platelet activation. Circulation 54 Suppl. II 197.
    PubMedGoogle Scholar
  • 10 MacMillan D. C. 1966; Secondary clumping effect in human citrated platelet rich plasma produced by adenosine diphosphate and adrenaline. Nature 211: 140.
    CrossrefPubMedGoogle Scholar
  • 11 Mills D. C. B, Robb I. A, Roberts G. C. K. 1968; Release of nucleotides, 5 hydroxytryptamine and enzymes from human blood platelets during aggregation. Journal of Physiology 195: 715.
    CrossrefPubMedGoogle Scholar
  • 12 Nugteren D. H, Beerthuis R. K, van Dorp D. A. 1966; The enzymatic conversion of all-cis, 8, 11, 14-eicosatrienoic add into prostaglandin E1 . Recueil des travaux chimiques des Pays-Bas 85: 405.
    PubMedGoogle Scholar
  • 13 Nugteren D. H, Hazelhof E. 1973; Isolation and properties of intermediates in prostaglandin biosynthesis. Biochimica et biophysica acta 326: 448.
    CrossrefPubMedGoogle Scholar
  • 14 Okuma M, Steiner M, Baldini M. G. 1970; Studies on lipid peroxides in platelets. I. Method of assay and effects of storage. Journal of Laboratory and Clinical Medicine 75: 283.
    PubMedGoogle Scholar
  • 15 Okuma M, Steiner M, Baldini M. G. 1971; Studies on lipid peroxides in platelets. II. Effect of aggregating agents and platelet antibody. Journal of Laboratory and Clinical Medicine 77: 728.
    PubMedGoogle Scholar
  • 16 Patton S, Kurtz C. 1957; 2-Thiobarbituric acid as a reagent for detecting milk fat oxidation. Journal of Dairy Science 34: 669.
    PubMedGoogle Scholar
  • 17 Robinson C. W, Mason R. G, Wagner R. H. 1963; Effect of sulphydryl inhibitors on platelet agglutinability. Proceedings of the Society of Experimental Biology and Medicine 133: 857.
    PubMedGoogle Scholar
  • 18 Samuelsson B. 1966; On the incorporation of oxygen in the conversion of 8, 11, 14-eicosatrienoic acid to prostaglandin. Journal of the American Chemical Society 87: 3011.
    PubMedGoogle Scholar
  • 19 Samuelsson B. 1977. The role of prostaglandin endoperoxides and thromboxanes in human platelets. In: Prostaglandins in Hematology. (eds.) Silver M. J, Smith J. B, Kocsis J. J. Spectrum; New York: 1.
    Google Scholar
  • 20 Sandler M, Karim S. M. M, Williams E. D. 1968; Prostaglandins in amine-peptide-secreting tumors. Lancet II: 1053.
    PubMedGoogle Scholar
  • 21 Shio H, Ramwell P. W. 1972; Effect of prostaglandin E2 and aspirin on the secondary aggregation of human platelets. Nature New Biology 236: 45.
    CrossrefPubMedGoogle Scholar
  • 22 Smith J. B, Willis A. L. 1970; Formation and release of prostaglandins by platelets in response to thrombin. British Journal of Pharmacology 40: 545
    PubMedGoogle Scholar
  • 23 Smith J. B, Willis A. L. 1971; Aspirin selectively inhibits prostaglandin production in human platelets. Nature New Biology 231: 235.
    CrossrefPubMedGoogle Scholar
  • 24 Smith J. B, Ingerman C, Kocsis J. J, Silver M. J. 1973; Formation of prostaglandins during aggregation of blood platelets. Journal of Clinical Investigation 52: 965.
    CrossrefPubMedGoogle Scholar
  • 25 Smith J. B, Ingerman C, Kocsis J. J, Silver M. J. 1974; Formation of an intermediate in prostaglandin biosynthesis and its association with the platelet release reaction. Journal of Clinical Investigation 53: 1468.
    CrossrefPubMedGoogle Scholar
  • 26 Smith J. B, Ingerman C. M, Silver M. J. 1976; Malondialdehyde formation as an indicator of prostaglandin production by human platelets. Journal of Laboratory and Clinical Medicine 88: 167.
    PubMedGoogle Scholar
  • 27 Smith J. B, Ingerman C. M, Silver M. J. 1977. Effects of arachidonic add and some of its metabolites on platelets. In: Prostaglandins in Hematology. (eds.) Silver M. J, Smith J. B, Kocsis J. J. Spectrum; New York: 277.
    Google Scholar
  • 28 Stuart M. J, Murphy S, Oski F. A. 1975; A simple non radioisotope technic for the determination of platelet life-span. New England Journal of Medicine 292: 1310.
    CrossrefPubMedGoogle Scholar
  • 29 Thomas D. P. 1967; Effect of catecholamines on platelet aggregation caused by thrombin. Nature 215: 298.
    CrossrefPubMedGoogle Scholar
  • 30 Willis A. L. 1974; Isolation of a chemical trigger for thrombosis. Prostaglandins 5: 1.
    CrossrefPubMedGoogle Scholar
  • 31 Weiss H. J, Aledort L. M. 1967; Impaired platelet/connective tissue reaction in man after asp’rin ingestion. Lancet II: 495.
    PubMedGoogle Scholar