Different Expression of Procoagulant Activity in Human Cancer Cells Cultured “In Vitro” or in Cells Isolated from Human Tumor Tissues

Summary

We studied in a homologous system the procoagulant activity of human tumor cells cultured “in vitro” (1402 primary melanoma, Me 7110/2 metastatic melanoma, Hep G2 hepatoma and GLC₁ small cell lung carcinoma) or of cells freshly isolatedfrom different human tumor tissues.

Tumor cells cultured “in vitro” possessed and released a factor VII dependent procoagulant activity, which was inhibitedby concanavalin A and unaffected by iodoacetamide or HgCl₂. The activity released by the cells of metastatic melanoma was higher than that released by the cells of the primary tumor. On the contrary, cancer cells isolated from tumor tissues possessed and released a factor VII independent activity which was inhibited by iodoacetamide or HgCl₂ and was not modified by concanavalin A. Therefore, different methods for the preparation of tumor cell suspensions have to be used for the study of tumor procoagulants, since their expression depends very largely on the source of tumor cells. Furthermore, cultured human tumor cells are not an appropriate model for the “in vivo” procoagulant effect of tumor cells.

• References

• 1 Muszbek L. Hemostasis and Cancer. Boca Raton: CRC Press; 1987
• 2 Sagripanti A, Carpi A, Zacharski LR. Cancer and Blood Coagulation. Pisa: ETS Editrice; 1990
• 3 Esumi N, Fan D, Fidler IJ. Inhibition of murine melanoma experimental metastasis by recombinant desulfatohirudin, a highly specific thrombin inhibitor. Cancer Res 1991; 51: 4549-4556
• 4 Nierodzik ML, Plotkin A, Kajumo F, Karpatkin S. Thrombin stimulates tumorplatelet adhesion in vitro and metastasis in vivo. J Clin Invest 1991; 87: 229-236
• 5 Mussoni L, Canforti G, Gambacorti-Passerini C, Alessio G, Pepe S, Vaghi M, Erba E, Amato G, Landoni F, Mangioni C, Morasca L, Semeraro N, Donatri MB. Procoagulant and fibrinolytic activity of human ovarian carcinoma cells in culture. Eur J Cancer Clin Oncol 1986; 22: 373-380
• 6 Szczepanski M, Bardadin K, Zawadzki J, Pypno W. Procoagulant activity of gastric, colorectal, and renal cancer is factor VII-dependent. J Cancer Res Clin Oncol 1988; 114: 519-522
• 7 Bastida E, Ordinas A, Escolar G, Jamieson GA. Tissue factor in microvesicles shed from U87MG human glioblastoma cells induces coagulation, platelet aggregation, and thrombogenesis. Blood 1984; 64: 177-184
• 8 Grignani G, Jamieson GA. Tissue factor-dependent activation of platelets by cells and microvesicles of SK-OS-10 human osteogenic sarcoma cell line. Invasion Metastasis 1987; 7: 172-182
• 9 Falanga A, Gordon SG. Isolation and characterization of cancer procoagulant: a cysteine proteinase from malignant tissue. Biochemistry 1985; 24: 5558-5567
• 10 Donati MB, Gambacorti-Passerini C, Casali B, Falanga A, Vannotti P, Fossati G, Semeraro N, Gordon SG. Cancer procoagulant in human tumor cells: evidence from melanoma patients. Cancer Res 1986; 46: 6471-6474
• 11 Grignani G, Falanga A, Pacchiarini L, Alessio MG, Zucchella M, Fratino P, Donati MB. Human breast and colon carcinomas express cysteine proteinase activities with pro-aggregating and pro-coagulant properties. Int J Cancer 1988; 42: 554-557
• 12 Van de Water L, Tracy PB, Aranson D, Mann KG, Dvorak F. Tumor cell generation of thrombin via prothrombinase assembly. Cancer Res 1985; 45: 5521-5525
• 13 Curatolo L, Alessio MG, Casali B, Falanga A, Donati MB, Semeraro N. Procoagulant activity of mouse transformed cells: different expression in freshly isolated or cultured cells. In vitro Cell Dev Biol 1988; 24: 1154-1158
• 14 Fossati G, Taramelli D, Balsari A, Bogdanovich G, Andreola S, Parmiani G. Primary but not metastatic human melanomas expressing DR antigens stimulate autologous lymphocytes. Int J Cancer 1984; 33: 591-597
• 15 Tramelli D, Fossati G, Mazzocchi A, Delia D, Ferrone S, Parmiani G. Classes I and II HLA and melanoma-associated antigen expression and modulation on melanoma cells isolated from primary and metastatic lesions. Cancer Res 1986; 46: 433-439
• 16 Knowless BB, Howe CC, Aden DP. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 1980; 209: 497-499
• 17 Falanga A, Shaw E, Donati MB, Consonni R, Barbui T, Gordon S. Inhibition of cancer procoagulant by peptidyl diazomethyl ketones and peptidyl solfonium salts. Thromb Res 1989; 54: 389-398
• 18 Pacchiarini L, Meloni F, Zucchella M, Mangiarotti P, Tacconi F, Ballabio P, Grignani G. Proaggregating and procoagulant activities of human mesothelioma tumor cells at different stages of “in vitro” culture. Haematologica 1991; 76: 392-397
• 19 Grignani G, Jamieson GA. Platelets in tumor metastasis: generation of adenosine diphosphate by tumor cells is specific but unrelated to metastatic potential. Blood 1988; 71: 844-849
• 20 Grignani G, Pacchiarini L, Ricetti MM, Dionigi P, Jemos V, Zucchella M, Fratino P. Mechanisms of platelet activation by cultured human cancer cells and cells freshly isolated from tumor tissues. Invasion Metastasis 1989; 9: 298-309
• 21 Zucchella M, Dezza L, Pacchiarini L, Meloni F, Tacconi F, Bonomi E, Grignani G, Notario A. Human tumor cells cultured “in vitro” activate platelet function by producing ADP or thrombin. Haematologica 1989; 74: 541-545
• 22 Mohanty D, Ghosh K, Das KC. Studies on the mechanism of synthesis and release of the procoagulant activity from leukemic cells. Med Oncol Tumor Pharmacother 1991; 8: 15-21