Summary
Dogs were fed varying levels of trans-AMCHA (trans-4-aminomethyl-cyclohexane-1-carboxylic
acid), a known inhibitor of the fibrinolytic enzyme system. Evidence that dialysis
against citrate-veronal buffer in the cold for 24 hrs effectively removes AMCHA from
plasma is presented. Determinations of antiactivator, antifibrinolysin, and proactivator
were carried out on both dialyzed and undialyzed samples. At 55 mg/kg, a moderate immediate decrease in urokinase excretion occurred which was maintained
throughout the time of drug administration. At higher levels, increases in antiactivator
and decreases in proactivator and euglobulin lytic activity occurred, the extent of
which could be roughly correlated with the amount of drug administered. No changes
in either fibrinogen or antifibrinolysin were seen. An assay for the antiurokinase
activity in canine plasma is described. The results are discussed in terms of the
possible function of the fibrinolytic system as revealed by administration of inhibitors.
