Platelet Ca²⁺ Homeostasis: Na⁺-Ca²⁺ Exchange in Plasma Membrane Vesicles

Summary

A vesicular plasma membrane-enriched fraction obtained from human platelets exhibited ⁴⁵Ca²⁺ uptake in exchange for intravesicular Na⁺. The rate of Ca²⁺ uptake was linear up to 4 sec. The apparent Km for Ca²⁺ was 22 pM and the V_max 280 pmol/mg/ sec. Ca²⁺ efflux from Ca²⁺ loaded vesicles was obtained upon dilution into a NaCl but not a KC1 medium. The extent of Ca²⁺uptake was monotonically increased as the pH increased from 6 to 9. Na⁺-Ca²⁺ exchange was shown to be electrogenic. Ca²⁺ uptake was distinguished from binding by the induction of Ca²⁺ release after A23187 addition. These findings support a role for Na⁺-Ca²⁺ exchange in human platelet Ca²⁺ transport.

Keywords

Platelets - Ca²⁺ homeostasis - Plasma membrane - Na⁺-Ca²⁺ exchange

• References

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