Acetylsalicylic Acid and Sodium Salicylate Inhibit LPS-lnduced NF-kB/c-Rel Nuclear Translocation, and Synthesis of Tissue Factor (TF) and Tumor Necrosis Factor alfa (TNF-a) in Human Monocytes

L T N Osnes; K B Foss; G B Joø; C Okkenhaug; Å-B Westvik; R Øvstebø; P Kierulf

doi:10.1055/s-0038-1650694

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 76(06): 0970-0976
DOI: 10.1055/s-0038-1650694

Original Article

Schattauer GmbH Stuttgart

Acetylsalicylic Acid and Sodium Salicylate Inhibit LPS-lnduced NF-kB/c-Rel Nuclear Translocation, and Synthesis of Tissue Factor (TF) and Tumor Necrosis Factor alfa (TNF-a) in Human Monocytes

L T N Osnes

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

K B Foss

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

G B Joø

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

C Okkenhaug

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

Å-B Westvik

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

R Øvstebø

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

,

P Kierulf

The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway

› Author Affiliations

Abstract

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Summary

We have investigated the effects of acetylsalicylic acid and sodium salicylate on the LPS-induced synthesis of the pro-coagulant protein tissue factor (TF) and the pro-inflammatory protein tumor necrosis fac-tor-a (TNF-a), as well as the prostaglandin PGE₂ in human monocytes. Both drugs dose-dependently inhibited LPS-induced TF and TNF-a synthesis at the mRNA and the protein level, and reduced PGE2 production. As evidenced by electro mobility shift assay (EMSA) and the use of a NF-kB prototypic probe, these drugs probably exert their inhibitory effects by interference with the nuclear translocation of NF-KB/c-Rel proteins. These data may expand the understanding of the anti-thrombotic and anti-inflammatory effects of these drugs when activation of monocytes occurs.

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References

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