Characterization of Fcγ Receptors on Human Megakaryocytes

Zhanhe Wu; Boban Markovic; Colin N Chesterman; Beng H Chong

doi:10.1055/s-0038-1650339

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1996; 75(04): 661-667
DOI: 10.1055/s-0038-1650339

Original Article

Schattauer GmbH Stuttgart

Characterization of Fc_γ Receptors on Human Megakaryocytes

Zhanhe Wu

The Centre for Thrombosis and Vascular Research, the University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Randwick, New South Wales, Australia

,

Boban Markovic

The Centre for Thrombosis and Vascular Research, the University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Randwick, New South Wales, Australia

,

Colin N Chesterman

The Centre for Thrombosis and Vascular Research, the University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Randwick, New South Wales, Australia

,

Beng H Chong

The Centre for Thrombosis and Vascular Research, the University of New South Wales and the Department of Haematology, Prince of Wales Hospital, Randwick, New South Wales, Australia

› Author Affiliations

Abstract

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Summary

Megakaryocyte and platelet Fc_γ receptors (FcR) are of importance in the pathophysiology of immune complex-mediated thrombocytopenias such as heparin-induced thrombocytopenia. In this study, Fc_γR proteins and mRNAs in normal human megakaryocytes were examined. Fc_γR proteins were studied with immunocytochemical staining, dual colour flow cytometry and immunoprecipitation using monoclonal antibodies against Fc_γR I, Fc_γR II and Fc_γR III. Fc_γR mRNAs were measured with biotinylated cDNA or oligonucleotide probes using a novel quantitative in situ hybridization technique. Using these techniques, Fc_γR II protein and mRNA, but not Fc_γR I and Fc_γR III proteins and transcripts were detected in megakaryocytes. Further, transcript analysis showed that megakaryocytes contain only the transcript of Fc_γR IIA gene but no transcripts of Fc_γR IIB nor Fc_γR IIC genes; Fc_γR IIA transcripts with and without the transmembrane (TM) exon are present in approximately equal proportions. In contrast, neutrophils and macrophages also contain Fc_γR IIA transcript but Fc_γR IIA transcript with the TM exon predominates suggesting cell lineage-specific Fc_γR IIA expression. Fc_γR IIA transcript lacking the TM exon predicts the presence of a potential soluble form of Fc_γR in platelets and megakaryocytes which may have a physiological role as it can compete with the membrane-bound Fc_γR IIA for binding of IgG-containing immune complexes and thus protect these cells from excessive binding and injurious effects of immune complexes.

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References

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