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Thromb Haemost 1996; 75(03): 481-487
DOI: 10.1055/s-0038-1650301
Original Article
Schattauer GmbH Stuttgart

Characterization of a cDNA Encoding Murine Coagulation Factor VII

Esohe Idusogie
1   The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA
,
Elliot Rosen
1   The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA
2   The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Jie-Ping Geng
1   The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA
,
Peter Carmeliet
2   The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Desirá Collen
2   The Center for Transgene Technology and Gene Therapy, Flanders Interuniversity Institute for Biotechnology, KU Leuven, Belgium
,
Francis J Castellino
1   The Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA
› Author Affiliations
Further Information

Publication History

Received 31 August 1995

Accepted after revision 13 December 1995

Publication Date:
27 July 2018 (online)

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Summary

The cDNA encoding murine coagulation factor VII (mfVII) was isolated and reconstructed from a Λ Zap cDNA library generated from murine liver mRNA. The cDNA contains 1903 nucleotides spanning 15 bases upstream of the 5’-translation initiation codon, an open reading frame of 1338 nucleotides, 550 nucleotides downstream of the first stop codon and a 3’ poly(A) tail. The translation product is composed of a 41-amino acid signal/propeptide region followed by a 405-residue mature protein. The latter is highly homologous to that of human, rabbit, bovine, Rhesus monkey, and canine fVII. All protein domains of hfVII are strictly conserved in mfVII.

 

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