Thromb Haemost 1996; 75(01): 101-106
DOI: 10.1055/s-0038-1650228
Original Article
Schattauer GmbH Stuttgart

Platelet Adhesion following the Administration of Tissue-type Plasminogen Activator and Its Reversal by Vitamin E in Rats

Chauying J Jen
1  The Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Huei-Ping Dong
1  The Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Hsiun-ing Chen
1  The Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Lih-Yuh C Wing
1  The Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Ming-Jer Tang
1  The Department of Physiology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Wen-Chang Chang
2  The Department of Pharmacology, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Ming T Lin
3  The Department of Biochemistry, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
,
Guey-Yueh Shi
3  The Department of Biochemistry, National Cheng-Kung University Medical College, Tainan, Taiwan, Republic of China
› Author Affiliations
Further Information

Publication History

Received 28 March 1995

Accepted after revision 04 October 1995

Publication Date:
10 July 2018 (online)

Summary

Thrombolytic therapy is known to induce platelet-related side effects. We used a parallel-plate flow chamber, which was connected to the femoral artery of the rat, to measure platelet adhesion ex vivo. A collagen-coated arterioarterial shunt between two carotid arteries was used to measure shunt patency duration as an index of antithrombotic efficacy. Tissue-type plasminogen activator (t-PA), vitamin E, and the combination of these two were intravenously administered for 60 min. Measurements were performed before drug administration, and at 30, 60, 120 min after the initiation of drug infusion. Our results indicated that (1) treatments with t-PA or t-PA/vitamin E prolonged the time to shunt occlusion at 30 and 60 min; (2) t-PA enhanced platelet adhesion at 60 and 120 min; (3) vitamin E tended to reduce platelet adhesion; (4) t-PA/vitamin E reduced the t-PA-enhanced platelet adhesion; (5) at the high-density area of platelet adhesion under t-PA treatment, the adherent platelets demonstrated severe morphological changes which could be blocked by vitamin E. These data suggest that t-PA may enhance platelet adhesion in rats and that this adverse effect can be suppressed by co-administration of vitamin E.