Effects of Dimethyl Sulphoxide and of Vitamin A on Human Platelets

M. G Davey; E. F Lüscher

doi:10.1055/s-0038-1650204

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(01/02): 012-017
DOI: 10.1055/s-0038-1650204

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Effects of Dimethyl Sulphoxide and of Vitamin A on Human Platelets

Authors

M. G Davey^*

¹Theodor Kocher Institute, University of Berne
E. F Lüscher

¹Theodor Kocher Institute, University of Berne

Abstract

Summary

Dimethyl sulphoxide (DMSO) causes some release of amino acids, adenine nucleotides, and acid phosphatase and β-glucuronidase activities from washed human platelets. Addition of vitamin A with DMSO greatly enhances the effect; but vitamin A in ethanol added to platelets fails to release amino acids and nucleotides. Release appears to be due to a change in permeability of the platelet membrane, not to the discharge of contents of intracellular structures. Lipid peroxidation of membrane phospholipids, or a similar reaction, may be involved. Thrombin causes a different type of alteration in platelet permeability.

The results suggest that DMSO should not be used as a preservative for platelets. They may have some bearing on the mechanisms of toxicity of DMSO and vitamin A.

Full Text

References

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