Thromb Haemost 1981; 45(03): 272-275
DOI: 10.1055/s-0038-1650186
Original Article
Schattauer GmbH Stuttgart

Heterogeneity of Molecular Size of Factor Vlll/von Willebrand Factor in von Willebrand's Disease

S Mikami
The Department of Pediatrics, Nara Medical College, Nara, Japan
,
Y Takahashi
The Department of Pediatrics, Nara Medical College, Nara, Japan
,
M Nishino
The Department of Pediatrics, Nara Medical College, Nara, Japan
,
Y Okubo
The Department of Pediatrics, Nara Medical College, Nara, Japan
,
H Fukui
The Department of Pediatrics, Nara Medical College, Nara, Japan
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Weitere Informationen

Publikationsverlauf

Received 02. Februar 1981

Accepted 21. April 1981

Publikationsdatum:
06. Juli 2018 (online)

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Summary

Patterns of VIIIR:AG in the plasma and its fractions, cryoprecipitate and cryosupernatant, from various types of von Willebrand’s disease (vWd) were observed by SDS 1.5% polyacrylamide gel electrophoresis - crossed immunoelectrophoresis (SDS PAGE - CIE).

VIIIR:AG in normal cryoprecipitate showed several precipitin peaks which correspond to molecular weights ranging from 8 X 105 to 1 X 10 7 daltons and are similar to those in normal plasma. Normal cryosupernatant VIIIR: AG gave smaller molecular weights from 8 X 10 5 to 2 X 10 6 daltons.

VIIIR:AG in the plasma and cryoprecipitate from 2 patients with classical vWd gave low precipitin peaks with molecular weights in normal range. VIIIR:AG from 2 patients with subgroup A variant which showed fast anodal migration on the conventional CIE, presented 3 peaks with molecular weights of 8 X 105 to 3 X 106 which are similar to those in normal cryosupernatant. VIIIR: AG from 2 patients with subgroup B variant which showed normal migration on the CIE, gave normal patterns through all fractions.