Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1980; 44(02): 100-104DOI: 10.1055/s-0038-1650093 Original Article Schattauer GmbH Stuttgart Kinetics and Fate of 111Indium-Oxine Labelled Blood Platelets in Asplenic Subjects Anthon duP Heyns Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa , Matthys G Lötter Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa , Philip N Badenhorst Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa , Otto van Reenen Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa , Henry Pieters Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa , Phil C Minnaar Departments of Haematology and Biophysics, University of the Orange Free State, Bloemfontein, South Africa› Author AffiliationsRecommend Article Abstract PDF Download Buy Article Summary The survival, tissue distribution and fate of 111Indium-oxine labelled autologous platelets was studied in four asplenic subjects with serial blood sampling, scintillation camera and computer-assisted imaging. Mean 111In-platelet recovery in the circulation was 89 ± 13% (± 1 SD). Platelet survival curves fitted a linear function best and was 238 ± 41 h. The shape of the survival curves of normal and asplenic subjects differed: in the asplenic subjects the curve was linear whereas that of normal subjects was significantly more curvilinear if analyzed by least squares computer fitting to a gamma function. Early hepatic 111In-activity was significant and transient and ascribed to the "collection injury". As labelled platelets disappeared from the circulation, 111Inactivity in the liver increased progressively and linearly to reach 42.5 ± 14.1 % of whole body activity at 240 h. Radioactivity also accumulated in the bone marrow, but could not be demonstrated in the vasculature of the lower limbs. These results would indicate that in asplenic subjects the major sites of destruction of senescent platelets are the liver and bone marrow. Key words Key words 111Indium-oxine - 111In-labelled blood platelets - Platelet kinetics in asplenic human subjects - Splenectomy, platelet kinetics after - Platelets, sites of destruction PDF (1291 kb) References References 1 Davey MG. The survival and destruction of human platelets. Bibl Haematol 1966; Fasc 22: 1-136 2 Kotilainen M. Platelet kinetics in normal subjects and haematological disorders with special reference to thrombocytopenia and to the role of the spleen. Scand J Haematol 1969; Suppl 5: 9-97 3 Aster RH. Studies of the fate of the platelets in rats and man. Blood 1969; 34: 117-128 4 Thakur ML, Welch MJ, Joist JH, Coleman RE. Indium-111-labeled platelets: studies on preparation and evaluation of in vitro and in vivo functions. Thromb Res 1976; 9: 345-357 5 McAfee JG, Thakur ML. Survey of radioactive agents for in vitro labeling of phagocytic leukocytes 1. Soluble agents J Nucl Med 1976; 17: 480-487 6 Heyns A duP, Lötter MG, Badenhorst PN, van Reenen OR, Pieters H, Minnaar PC, Retief FP. Kinetics, distribution and sites of destruction of 111Indium-labeled human platelets. Br J Haematol 1980; 44: 269-280 7 Lötter MG, Badenhorst PN, Heyns AduP, van Reenen OR, Pieters H, Minnaar PC. Kinetics, distribution and sites of destruction of In-111-oxine labeled canine platelets. J Nucl Med 1980; 21: 36-40 8 Bull BS, Schneiderman MA, Brecher G. Platelet counts with the Coulter Counter. Am J Clin Pathol 1965; 44: 678-688 9 Heyns AduP, Badenhorst PN, Pieters H. Preparation of a viable population of Indium-111-labeled human blood platelets. Thromb Haemostas 1980; 42: 1473-1482 10 Panel on diagnostic Application of Radioisotopes in Hematology Recommended methods for radioisotope platelet survival studies. Blood 1977; 50: 1137-1144 11 Murphy EA, Francis ME. The estimation of blood platelet survival. II The multiple hit model. Thromb Diath Haemorrh 1971; 25: 53-80 12 Nadler SB, Hidalgo JU, Bloch T. Prediction of blood volume in normal human adults. Surgery 1962; 51: 224-232 13 Aster RH. Pooling of platelets in the spleen: Role in the pathogenesis of "hypersplenic" thrombocytopenia. J Clin Invest 1966; 45: 645-657 14 Harker LA, Finch CA. Thrombokinetics in man. J Clin Invest 1969; 48: 963-974 15 Ljungqvist U, Bergentz SE. The effect of experimental trauma on the platelet. Acta Chir Scand (Suppl) 1970; 411: 1-40 16 Baldini M. Platelet circulation and survival. Bibl Haemat 1966; 29: 107-122 17 Cronkite EP, Bond VP, Fliedner TM, Paglia DA Adamiker. Studies on the origin, production and destruction of platelets. Tenth International Henry Ford Hospital Symposium 1961; 595-609 18 Cohen P, Gardner FU, Barnett CO. Reclassification of the thrombocytopenias by the 51Cr-labelling method for measuring platelet life span. New Eng J Med 1961; 264: 1294-1350 19 Najean Y, Ardaillou N, Caen J, Larrieu MJ, Bernard J. Survival of radiochromium-labelled platelets in thrombocytopenias. Blood 1963; 22: 718 20 Abrahamsen AF. Platelet survival studies in man – with special reference to thrombosis and atherosclerosis. Scand J Haematol (Suppl) 1968; 3: 7-53 21 Ritchie JL, Harker LA. Platelet and fibrinogen survival in coronary atherosclerosis: Response to medical and surgical therapy. Am J Cardiol 1977; 39: 595-598
