Studies of Aggregation and Thromboxane B₂ Synthesis in Avian Thrombocytes: Comparison between Atherosclerosis-Susceptible and Resistant Breeds

Summary

Comparison of thrombocyte aggregation in response to arachidonic acid and synthesis of prostaglandins from C¹⁴-arachidonic acid by thrombocytes was made between atherosclerosis-susceptible White Carneau and atherosclerosis-resistant Show Racer Pigeons. Contrary to the expectations, thrombocytes from atherosclerosis-susceptible pigeons were a) less sensitive to arachidonic acid induced aggregation and b) synthesized less thromboxane B₂ (stable product of proaggregatory thromboxane A₂) from C¹⁴-arachidonic acid. Analysis of fatty acid composition of thrombocyte phospholipids indicated a higher concentration of arachidonic acid in White Carneau Pigeons. These results suggest that thrombocytes from atherosclerosis-susceptible pigeons are altered early and as a defense mechanism display less responsiveness towards aggregation.

Keywords

Avian thrombocytes - Thromboxane B₂ - Thrombocyte aggregation - Atherosclerosis-susceptibility

• References

• 1 French JE. Atherogenesis and Thrombosis. Seminars in Haematology 1971; 8: 84-94
• 2 Mustard JF. Packham The role of blood and platelets in atherosclerosis and complications of atherosclerosis. Thromb. Diath. Haemorrh 1975; 33: 448-456
• 3 Ross R and HarkerL. Hyperlipidemia and atherosclerosis. science 1976; 193: 1094-1100
• 4 Ross R and Glomset JA. Pathogenesis of atherosclerosis. New Eng J Med, 1976; 29: 369-420
• 5 Carvalho AC, Colemann R W and Lees RS. Platelet Function in hyperbetalipoproteinemia. New Eng J Med 1976; 290: 434-438
• 6 Colwell JA, Nair RM G, Halushka PV, Rogers C. et al Platelet adherance and aggregation in diabetes mellitus. Metabolism 1979; 28 (Suppl 1) 394-402
• 7 Moncada S and Vane JR. Mode of action of aspirin like drugs. Advan Inter Med 1979; 24: 1-25
• 8 Roth GJ, Stanford N and Majerus PW. Acetylation of prostaglandin synthetase by aspirin. Proc Nat Acad Sci, 1975; 72: 3073-3076
• 9 Elwood PC, Cochrane AL, Burn ML, et al. A randomized controlled trial of acetylsalcylic acid in the secondary prevention of mortality from myocardial infarction. Brit Med J, 1976; 1: 436
• 10 Pick R, Chediak J and GlickC. Aspirin inhibits development of coronary atherosclerosis in Cynomolgas monkeys fed an atherogenic diet. J Clin Invest 1979; 63: 158-162
• 11 Subbiah MT R. White Carneau Pigeon: An ideal model for the study of progression and regression of atherosclerosis. Biochem Exptl Biol, 1976; 12: 229-243
• 12 Lewis JC, Fuster V and Kottke BA. Thrombocytes in atherosclerosis-resistant and atherosclerosis-susceptible bread of pigeon: A comparative study. Expt Mol Path 1976; 25: 332-343
• 13 Hamberg M, Svensson J and SamuelssonB. Thromboxane: A new group of biologically active compounds derived from prostaglandins endo periods. Proc Nat Acad Sci, 1975; 72: 2994-2998
• 14 Subbiah MT R. Prostaglandin E₂ biosynthesis and effect in pigeon aorta. Possible role in atherogenesis Atheroslerosis 1978; 29: 487-495
• 15 Transik RL, Namm D H and White HL. Synthesis of prostaglandin 6-keto F_{1 α} by cultured aortic smooth muscle cells and stimmulation of its formation in a coupled system with platelet lysates. Prostaglandins, 1978; 15: 399-408
• 16 Subbiah MT R. On the characterization of PGE₂ 9-keto reductase from aorta: Proc. Soc. Expt. Biol. Med. 1979; 161: 158-161
• 17 Lowry OR, Rosenbrough NJ, Farr AG, and Randall PJ. Protein measurement with folin-phenol reagent. J Biol Chem 1951; 193: 205-215
• 18 Mellies MJ, Ishikawa TT, Gartside PS, Burton KS. et al (1979) Effect of varying material dietary fatty acid in lactating women and their infants. Amer J Clin Nutr 1979; 32: 299-309
• 19 Zmuda A, Diembinska A, Chytowski A, Gryglewski RJ. Experimental atherosclerosis in rabbits – platelet aggregation, Thromboxane A₂ generation and anti-aggregatory potency of prostacyclin. Prostaglandins 1977; 16: 1035-1045
• 20 Lewis J C and Kottke BA. Endothelial damage and thrombocyte adhesion in pigeon atherosclerosis. Science 1977; 196: 1007-1009