Simplified Thromboplastin Calibration: Further Experience of the Dutch Reference Laboratory for Anticoagulant Control

Summary

The modified (and thereby simplified) thromboplastin calibration procedure using pooled lyophilized normal and patient plasmas is shown to equal, for batch-to-batch control of Thrombotest, the calibration procedure described by Biggs and Denson, which makes use of individual freshly prepared patient plasmas.

Keywords

Thromboplastin calibration - Thrombotest - Anticoagulation control - Lyophilized plasmas

• References

• 1 Biggs R, Denson KW E. Standardization of the one-stage prothrombin time for the control of anticoagulant therapy. Brit Med J 1967; 1: 84-88
• 2 Loeliger EA, van Halem-Visser LP. A simplified thromboplastin calibration procedure for standardization of anticoagulant control. Thrombos Diathes haemorrh 1975; 33: 172-190
• 3 ICSH/ICTH Prothrombin time standardization. Report of the Expert Panel on Oral Anticoagulant Control. The International Committee on Thrombosis and Haemostasis. The International Committee on Standardization in Haematology. Thrombos Haemostas 1979; 42: 1073
• 4 Loeliger EA, van Halem-Visser LP. Results of calibration by the Dutch National Reference Laboratory of the thromboplastins included in the ICTH/ICSH collaborative study of prothrombin time standardization. Thrombos Haemostas 1979; 42: 1128
• 5 Loeliger EA, van der Hoeff-van Halem R, van Halem-Visser LP. Thromboplastin calibration. Experience of the Dutch Reference Laboratory for Anticoagulant Control. Thrombos Haemostas 1978; 40: 272-287