Download PDF
Thromb Haemost 1980; 43(01): 034-037
DOI: 10.1055/s-0038-1650006
Original Article
Schattauer GmbH Stuttgart

The Lysis of Artificial Thrombi

Michael J Metcalf
The Department of Experimental Medicine, Pharmacia A. B., Uppsala, Sweden and Department of Surgery, Ninewells Hospital and Medical School, Dundee, Scotland
› Author Affiliations
Further Information

Publication History

Received 20 August 1979

Accepted 21 November 1979

Publication Date:
13 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

The susceptibility to lysis of artificial thrombi formed from native rabbit blood in the presence or absence of dextran was determined using the Chandler loop technique. Thrombi of identical weight were formed in the presence of saline or dextran and when spontaneous thrombolysis was allowed to take place, thrombi formed in the presence of dextran 70 were lysed to a greater extent than those formed under control conditions.

The possible factors influencing this observation were studied. Increasing concentrations of streptokinase increased the extent of thrombolysis in both control and dextran treated thrombi. Maximal streptokinase induced thrombolysis occurred in the presence of a 2 per cent final concentration of dextran Mw 40,000 and 500,000 and dextran 70 at a final concentration of 1.2 per cent.

Increased thrombolysis was not observed when albumin was substituted for dextran.

Finally, similar observations were recorded for streptokinase induced thrombolysis using human blood.

Keywords

Thrombolysis - Chandler loop - Dextran 70
 

  • References

  • 1 Chandler AB. In vitro thrombotic coagulation of the blood. Lab Invest 1958; 7: 110-114
    PubMedGoogle Scholar
  • 2 Jacobsen CD, Chandler AB. Thrombolysis in vitro. 1. Method, comparison of various thrombolytic agents and factors influencing thrombolysis. Scand J Clin & Lab Invest 1965; 17 Suppl 84: 209-225
    CrossrefPubMedGoogle Scholar
  • 3 Krøll J. Studies on the effect of low molecular weight dextran upon the clotting process in vitro . Scand J Clin & Lab Invest 1965; 17: 51-56
    CrossrefPubMedGoogle Scholar
  • 4 Gollub S, Schaefer C. Structural alteration in canine fibrin produced by colloid plasma expanders. Surg Gyn & Obst 1968; 127: 783-793
    PubMedGoogle Scholar
  • 5 Muzaffar TZ, Stalker AL, Bryce WA J, Dhall DP. Dextrans and fibrin morphology. Nature 1972; 238: 288-290
    CrossrefPubMedGoogle Scholar
  • 6 Tangen O, Wik KO, Almqvist IA M, Arfors KE, Hint HC. Effects of dextran on the structure and plasmin-induced lysis of human fibrin. Thrombosis Res. 1972; 1: 487-492
    CrossrefPubMedGoogle Scholar
  • 7 Tillet WS, Garner RL. The fibrinolytic activity of haemolytic streptococci. J Exp Med 1933; 58: 485-502
    CrossrefPubMedGoogle Scholar
  • 8 Jannach JR, Fuerst DE. The effect of human, rabbit and guinea-pig serum on local streptococcal infection and its relation to the streptokinase-plasminogen system. J Path & Bact 1965; 89: 402-406
    CrossrefPubMedGoogle Scholar
  • 9 Hirsch J, Buchanan M, Glynn MF, Mustard JF. Effect of activation of the fibrinolytic mechanism on experimental platelet-rich thrombi in rabbits. J Lab & Clin Med 1968; 72: 245-255
    PubMedGoogle Scholar