Thromb Haemost 1995; 74(04): 1169-1174
DOI: 10.1055/s-0038-1649898
Original Article
Vessel Wall
Schattauer GmbH Stuttgart

Minimum Fragments of the Heparin Molecule Able to Produce the Accumulation and Change of the Sulfation Pattern of an Antithrombotic Heparan Sulfate from Endothelial Cells

Maria A S Pinhal
The Departamento de Bloquímlca Escola Paulista de Medicina, Sao Paulo, Brazil
,
Isabel A N Santos
The Departamento de Bloquímlca Escola Paulista de Medicina, Sao Paulo, Brazil
,
Irani F Silva
The Departamento de Bloquímlca Escola Paulista de Medicina, Sao Paulo, Brazil
,
Carl P Dietrich
The Departamento de Bloquímlca Escola Paulista de Medicina, Sao Paulo, Brazil
,
Helena B Nader
The Departamento de Bloquímlca Escola Paulista de Medicina, Sao Paulo, Brazil
› Institutsangaben
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Publikationsverlauf

Received 27. Januar 1995

Accepted after resubmission 23. Juni 1995

Publikationsdatum:
09. Juli 2018 (online)

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Summary

Heparin and low molecular weight heparins stimulate two to three fold the accumulation of an antithrombotic heparan sulfate secreted by endothelial cells in culture. This led us to search for the minimum structural requirements of the heparin molecule able to elicit the enhancement of the heparan sulfate. Fragments were prepared from heparin by degradation with bacterial heparinase and heparitinases. A heparin pentasulfated tetrasaccharide was shown to be the minimum structural sequence able to enhance two to three fold the secretion of heparan sulfate by endothelial cells. The stimulation is specific for the endothelial cell, is concentration dependent and the effect is already noticed afterone hour of exposure of the cells to heparinand the tetrasaccharide. Degradation of the [35S]-heparan sulfate synthesized in the presence of heparin or the tetrasaccharide has shown a higher degree of sulfation of its iduronic acid residues.