Assembly and Activation of the Intrinsic Fibrinolytic Pathway on the Surface of Human Endothelial Cells in Culture

Catherine Lenich; Ralph Pannell; Victor Gurewich

doi:10.1055/s-0038-1649800

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1995; 74(02): 698-703
DOI: 10.1055/s-0038-1649800

Original Article

Fibrinolysis

Schattauer GmbH Stuttgart

Assembly and Activation of the Intrinsic Fibrinolytic Pathway on the Surface of Human Endothelial Cells in Culture

Authors

Catherine Lenich

The Department of Medicine, Vascular Research Laboratory, Institute for the Prevention of Cardiovascular Disease, Deaconess Hospital, Harvard Medical School, Boston, USA
Ralph Pannell

The Department of Medicine, Vascular Research Laboratory, Institute for the Prevention of Cardiovascular Disease, Deaconess Hospital, Harvard Medical School, Boston, USA
Victor Gurewich

The Department of Medicine, Vascular Research Laboratory, Institute for the Prevention of Cardiovascular Disease, Deaconess Hospital, Harvard Medical School, Boston, USA

Abstract

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Summary

Factor XII has long been implicated in the intrinsic pathway of fibrinolysis, but the mechanism by which it triggers plasminogen activation and targets fibrinolysis has not been established. In the present study, the assembly and function of activated Factor XII (F.XIIa), prourokinase (pro-u-PA), high molecular weight kininogen (H-kininogen), and prekallikrein on human umbilical vein endothelial cells (HUVEC) was investigated. ¹²⁵I-prekallikrein was shown to bind to HUVEC via receptor-bound H-kininogen in the presence of 50 μM ZnCl₂. After the addition of F.XIIa, 78% of the ¹²⁵I-prekallikrein initially bound to HUVEC was converted to ¹²⁵I-kallikrein. However, only 6% of the HUVEC-bound ¹²⁵I-pro-u-PA was thereby activated. This discrepancy was shown to be related to rapid dissociation (>50% within 15 min) of prekallikrein/kallikrein, but not pro-u-PA, from HUVEC. Increasing the level of cell-bound kallikrein increased the portion of cell-bound pro-u-PA activated, indicating that their co-localization was important for this pathway. Finally, F.XIIa was shown to trigger plasminogen activation on HUVEC via this pathway. This assembly of reactants on the endothelium suggests a mechanism whereby local fibrinolysis may be triggered by blood coagulation.

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References

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