Download PDF
Thromb Haemost 1993; 70(03): 514-521
DOI: 10.1055/s-0038-1649615
Original Article
Platelets
Schattauer GmbH Stuttgart

The Platelet Glycoprotein IIb/IIIa Complex Is lnvolved in the Adhesion of Activated Platelets to Leukocytes

Peter Spangenberg
The Institute of Pathological Biochemistry, University of Münster, FRG
,
Helge Redlich
The Institute of Pathological Biochemistry, University of Münster, FRG
,
Iris Bergmann
The Institute of Pathological Biochemistry, University of Münster, FRG
,
Wolfgang Lösche
The Institute of Pathological Biochemistry, University of Münster, FRG
,
Matthias Götzrath
1   Dept. of Clinical Chemistry of the School of Medicine of Erfurt, University of Münster, FRG
,
Beate Kehrel
2   Dept. of Internal Medicine, University of Münster, FRG
› Author Affiliations
Further Information

Publication History

Received 29 July 1992

Accepted after revision 18 March 1993

Publication Date:
05 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

The adhesion of activated platelets to leukocytes (rosette formation) seems to be mediated by CD62 on platelets and its counterreceptor (CD 15 or a sialic acid-containing glycoprotein) on polymorphonuclear leukocytes (PMNL). However, neither treatment of platelets with an anti-CD62 antibody or fucoidan nor treatment of PMNL with anti-CD15 antibody or neuraminidase are able to inhibit completely the adhesion. Therefore, we have studied the platelet GPIIb/IIIa complex (CD41a) for its involvement in the adhesion of activated platelets to PMNL. The following evidences point to a participation of CD41a in the adhesion of activated platelets to leukocytes: a) inhibition of adhesion by monoclonal antibodies (mab) raised toward CD41a, b) inhibition of adhesion by peptides such as RGDS and echistatin, c) inhibition of adhesion by dissociation of the CD41a complex with EGTA, and d) inhibition of rosette formation using platelets from a thrombasthenic patient which have almost no CD41a in the surface membrane but a normal expression of CD62. It is likely that fibrinogen is involved in the adhesion of platelets to PMNL via CD41a, since fibrinogen increases the rosette formation of ADP-stimulated platelets. Furthermore, the incubation of unstimulated platelets with fibrinogen and an antibody raised against glycoprotein III a which stimulates fibrinogen binding to the platelet surface results in an enlarged rosette formation.

 

  • References

  • 1 Field EJ, MacLeod I. Platelet adherence to polymorphs. Br Med J 1963; ii: 388-389
    PubMedGoogle Scholar
  • 2 Kjelsberg CR, Swanson J. Platelet satellitism. Blood 1974; 42: 831-836
    PubMedGoogle Scholar
  • 3 Ravel R, Bassart JA. Platelet satellitosis and phagocytosis by leukocytes. Lab Med 1974; 5: 41-42
    PubMedGoogle Scholar
  • 4 Reisman LE, Sabesin SS. Platelet satellitism. N Engl J Med 1974; 290: 691
    PubMedGoogle Scholar
  • 5 Larson JH, Pierre RV. Platelet satellitism as a cause of abnormal hemalog D differential results. Am J Clin Pathol 1977; 68: 758-759
    CrossrefPubMedGoogle Scholar
  • 6 Henry RL. Leukocytes and thrombosis. Thromb Diath Haemorrh 1961; 35: 13-25
    PubMedGoogle Scholar
  • 7 Webster J, Sixma JJ, Geuze JJ, Heijnen HFG. Morphology of the hemostatic plug in human skin wounds. Transformation of the plug. Lab Invest 1979; 41: 182-189
    PubMedGoogle Scholar
  • 8 Issekutz AC, Ripley M, Jackson JR. Role of neutrophils in the deposition of platelets during acute inflammation. Lab Invest 1983; 49: 716-724
    PubMedGoogle Scholar
  • 9 Bednar M, Smith B, Pinto A, Mullane K. Neutrophil depletion suppresses 111-labelled platelet accumulation in infarcted myocardium. J Cardiovasc Pharmacol 1985; 7: 906-912
    CrossrefPubMedGoogle Scholar
  • 10 Weksler BB. Platelets and the inflammatory response. Clin Lab Med 1983; 3: 667-676
    CrossrefPubMedGoogle Scholar
  • 11 Jungi TW, Spycher MO, Nydegger UE, Barandun S. Platelet-leukocyte interaction: selective binding of thrombin-stimulated platelets to human monocytes, polymorphonuclear leukocytes, and related cell lines. Blood 1986; 67: 629-636
    PubMedGoogle Scholar
  • 12 Larsen E, Celi A, Gilbert GE, Furie BC, Erban JK, Bonfanti R, Wagner DD, Furie B. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell 1989; 59: 305-312
    CrossrefPubMedGoogle Scholar
  • 13 Hamburger SA, McEver RP. GMP-140 mediates the adhesion of stimulated platelets to neutrophils. Blood 1990; 75: 550-554
    CrossrefPubMedGoogle Scholar
  • 14 Larsen E, Palabrica T, Sajer S, Gilbert GE, Wagner DD, Furie BC, Furie B. PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15). Cell 1990; 63: 467-484
    CrossrefPubMedGoogle Scholar
  • 15 Moore KL, Varki A, McEver RP. GMP-140 binds to a glycoprotein receptor on human neutrophils: evidence for a lectin-like interaction. J Cell Biol 1991; 112: 491-499
    CrossrefPubMedGoogle Scholar
  • 16 Spangenberg P, Schymik Ch, Hofmann B, Ostermann G, Rühling K, Till U. Blood platelet behaviour in patients with a type I diabetes mellitus. Exp Endocrinol 1989; 94: 329-337
    PubMedGoogle Scholar
  • 17 Lösche W, Michel E, Spangenberg P, Thielmann K. Eine einfache und empfindliche Methode zur Bestimmung der Phagozytoseaktivität humaner polymorphkerniger Leukozyten. Z Med Lab Diagn 1987; 28: 369-373
    PubMedGoogle Scholar
  • 18 Geng J-G, Bevilacqua MP, Moore KL, Mclntyre TM, Prescott SM, Kim JM, Bliss GA, Zimmerman GA, McEver RP. Rapid adhesion to activated endothelium mediated by GMP-140. Nature 1990; 343: 757-760
    CrossrefPubMedGoogle Scholar
  • 19 Corral L, Singer MS, Macher BA, Rosen SD. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets. Biochem Biophys Res Commun 1990; 172: 1349-1356
    CrossrefPubMedGoogle Scholar
  • 20 Kouns WC, Wall CD, White MM, Fox CF, Jennings LK. A conformation-dependent epitope of human platelet glycoprotein III a. J Biol Chem 1990; 265: 20594-20601
    PubMedGoogle Scholar
  • 21 Silverstein RL, Asch AS, Nachman RL. Glycoprotein IV mediates thrombospondin-dependent platelet-monocyte and platelet-U937 cell adhesion. J Clin Invest 1989; 84: 546-552
    CrossrefPubMedGoogle Scholar
  • 22 Parmentier S, McGregor L, Catimel B, Leung LLK, McGregor JL. Inhibition of platelet functions by a monoclonal antibody (LYP 20) directed against a granule membrane glycoprotein (GMP-140/ PADGEM). Blood 1991; 177: 1734-1739
    PubMedGoogle Scholar
  • 23 Aruffo A, Dietsch MT, Wan H, Hellström KE, Hellström I. Granule membrane protein 140 (GMP-140) binds to carcinomas and car-cinoma-derived cell lines. Proc Natl Acad Sci USA 1992; 89: 2292-2296
    CrossrefPubMedGoogle Scholar
  • 24 McEver RP. Selectins: novel receptors that mediate leukocyte adhesion during inflammation. Thromb Haemostas 1991; 65: 223-228
    PubMedGoogle Scholar
  • 25 Rinder HM, Bonan JL, Rinder CS, Ault KA, Smith BR. Dynamics of leukocyte-platelet adhesion in whole blood. Blood 1991; 78: 1730-1737
    PubMedGoogle Scholar
  • 26 Krause S, Redlich H, Bergmann I, Spangenberg P, Lösche W. Blood platelets stimulate the chemiluminescence response of polymorphnuclear leukocytes via cell-cell contact. Fresenius J Anal Chem 1992; 343: 177-178
    CrossrefPubMedGoogle Scholar
  • 27 de Bruijne-Admiraal LG, Modderman PW, Von dem Borne AEGKr, Sonnenberg A. P-selectin mediates Ca2+-dependent adhesion of activated platelets to many different types of leukocytes: detection by flow cytometry. Blood 1992; 80: 134-142
    PubMedGoogle Scholar
  • 28 Skinner MP, Lucas CM, Bums GF, Chesterman CN, Berndt MC. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem 1991; 266: 5371-5374
    CrossrefPubMedGoogle Scholar
  • 29 Handa K, Nudelman ED, Stroud MR, Shiozawa T, Hakomori S. Selectin GMP-140 (CD62; PADGEM) binds to sialosyl-Lea and sialosyl-Lex, and sulfated glycans modulate this binding. Biochem Biophys Res Commun 1991; 181: 1223-1230
    CrossrefPubMedGoogle Scholar
  • 30 Zhou Q, Moore KL, Smith DF, Varki A, McEver RP, Cummings RD. The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells. J Cell Biol 1991; 115: 557-564
    CrossrefPubMedGoogle Scholar
  • 31 Polley MJ, Phillips ML, Wayner E, Nudelman E, Singhal AK, Hakomori S, Paulson JC. CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proc Natl Acad Sci USA 1991; 88: 6224-6228
    CrossrefPubMedGoogle Scholar
  • 32 McGregor JL, Catimel B, Parmentier S, Clezardin P, Dechavanne M, Leung LLK. Rapid purification and partial characterization of human platelet glycoprotein IIIb. Interaction with thrombospondin and its role in platelet aggregation. J Biol Chem 1989; 264: 501-506
    PubMedGoogle Scholar
  • 33 Gralnick HR, Williams S, McKeown L, Connaghan G, Shafer B, Hansmann K, Vail M, Fenton J. Endogenous platelet fibrinogen surface expression on activated platelets. J Lab Clin Med 1991; 118: 604-613
    PubMedGoogle Scholar
  • 34 Courtois G, Ryckewaert J-J, Woods VL, Ginsberg MH, Plow EF, Marguerie GA. Expression of intracellular fibrinogen on the surface of stimulated platelets. Eur J Biochem 1986; 159: 61-69
    CrossrefPubMedGoogle Scholar
  • 35 Zamarron C, Ginsberg MH, Plow EF. A receptor-induced binding site in fibrinogen elicited by its interaction with platelet membrane glycoprotein IIb-IIIa. J Biol Chem 1991; 266: 16193-16199
    PubMedGoogle Scholar
  • 36 Lorant DE, Patel KD, McIntyre TM, McEver RP, Prescott SM, Zimmermann GA. Coexpression of GMP-140 and PAF by endothelium stimulated by histamine or thrombin: a juxtacrine system for adhesion and activation of neutrophils. J Cell Biol 1991; 115: 223-234
    CrossrefPubMedGoogle Scholar
  • 37 Hirafuji M, Shinoda H. PAF-mediated platelet adhesion to endothelial cells induced by FMLP-stimulated leukocytes. J Lipid Med 1991; 4: 347-352
    PubMedGoogle Scholar
  • 38 Ninio E, Leyravaud S, Bidault J, Jurgens P, Benveniste J. Cell adhesion by membrane-bound paf-acether. Int Immuno 1991; 11: 1157-1163
    PubMedGoogle Scholar
  • 39 Lösche W, Redlich H, Krause S, Bergmann I, Spangenberg P. Platelets activate neutrophils via cell-cell contact. Ann Hematol 1992; 64: A14
    PubMedGoogle Scholar