Thromb Haemost 1993; 70(03): 423-426
DOI: 10.1055/s-0038-1649598
Original Article
Coagulation
Schattauer GmbH Stuttgart

Evidence that the Protein C Activation Pathway Amplifies the lnhibition of Thrombin Generation by Recombinant Human Thrombomodulin in Plasma

Rika ohishi
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Naoko watanabe
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Masaharu Aritomi
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Komakazu Gomi
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Takao Kiyota
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Shuji Yamamoto
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Torao lshida
1   The institute for Life Science Research, Asahi Chemical Industry, Fuji City, Japan
,
Ikuro Maruyama
2   The Department of Clinical Laboratory Medicine, Kagoshima University School of Medicine, Kagoshima City, Japan
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 16. Juli 1992

Accepted after revision 12. März 1993

Publikationsdatum:
05. Juli 2018 (online)

Summary

Thrombomodulin (TM) is a cofactor for the thrombin-catalyzed activation of anticoagulant protein C. However, we have no evidence that thrombomodulin actually activates protein C during blood coagulation processing, nor do we know whether this activated protein C acts as an anticoagulant. We studied the inhibitory action of recombinant human soluble TM (rhs-TM) on thrombin generation in whole plasma. Human plasma was activated with small amounts of tissue factor using phospholipid vesicles in place of activated platelets. Thrombin generation was observed. The addition of only 2 nM of rhs-TM prevented rapid generation of thrombin and reduced the total amount of thrombin generated. In order to study the influence of the protein C activation pathway on this inhibitory action of rhs-TM, protein C-depleted plasma was used. rhs-TM had little inhibitory effect on protein C-depleted plasma. However, the addition of protein C caused a delay in thrombin generation and a reduction of the maximum thrombin concentration. We concluded that the anticoagulant activity of rhs-TM was amplified by the protein C activation pathway.

 
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