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Thromb Haemost 1972; 27(03): 610-618
DOI: 10.1055/s-0038-1649400
Originalarbeiten — Original Articles — Travaux Originaux
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Identification of Fibrinogen Derivatives in Plasma Samples

H Graeff
1   First Department of Obstetrics and Gynecology (Head: Prof. Dr. J. Zander), Munich University, Germany
,
R von Hugo   *
1   First Department of Obstetrics and Gynecology (Head: Prof. Dr. J. Zander), Munich University, Germany
› Author AffiliationsThis study was supported by DFG, “Sonderforschungsbereich 51, München”, grant no. 2/Gra
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Publication Date:
29 June 2018 (online)

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Summary

The observation of fibrinogen derivatives with a molecular weight higher than the parent molecule in human cases of DIC initiated the present methodological study. These derivatives were identified by the following methods : 2.5 M β-alanine precipitation of the plasma samples, PAA gel electrophoresis, intra gel immunoprecipitation and agarose gel chromatography. In the plasma of a patient with severe eclampsia and laboratory signs of DIC two derivatives with a molecular weight higher than that of fibrinogen were identified according to their relative electrophoretic mobilities: 0.18 and 0.28 × 10−5 cm2/V × sec (fibrinogen: 0.43 × 10−5 cm2/V × sec). Electrophoretic studies in the presence of 5 M urea indicated that the 0.28 derivative is a complex probably formed by fibrinogen and a fibrin monomer.

* DFG- Research Trainee, SFB 51.


 

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