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Thromb Haemost 1972; 27(03): 529-534
DOI: 10.1055/s-0038-1649392
Originalarbeiten — Original Articles — Travaux Originaux
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A Quantitative Two-Stage Assay for Factor IX (Christmas Factor) Using Plasma from Dogs with Christmas Disease

D. L Aronson
1   Division of Biologics Standards National Institutes of Health, Bethesda, Md., and Division of Laboratories New York State Department of Health, Albany, New York
,
W. J Dodds
1   Division of Biologics Standards National Institutes of Health, Bethesda, Md., and Division of Laboratories New York State Department of Health, Albany, New York
,
A. J Mustafa
1   Division of Biologics Standards National Institutes of Health, Bethesda, Md., and Division of Laboratories New York State Department of Health, Albany, New York
› Author Affiliations
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Publication History

Publication Date:
29 June 2018 (online)

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Summary

A modified 2-stage assay for factor IX which utilizes factor IX deficient plasma plasma from a colony of dogs with Christmas factor deficiency, has been described. The assay is technically less difficult and less time consuming than the established 2-stage assay for factor IX. The assay is more sensitive than other methods currently in use and uses only 4 μl of deficient plasma for a test. Results are comparable to those reported for 1-stage assays, both in vitro and in vivo, with an apparent increase in activity during the conversion of plasma to serum, and with apparently low in vivo recovery of factor IX when infused into a deficient patient.

 

  • References

  • 1 Langdell R. D, Wagner R. H, Brinkhous K. M. Effect of Antihemophilic Factor on one- stage clotting tests. J. Lab. clin. Med 41: 637 1953;
    PubMedGoogle Scholar
  • 2 Sen N. N, Sen R, Denson K. W. E, Biggs R. A method for the assay of Factor IX. Throm- bos. Diathes. haemorrh. (Stuttg) 18: 241 1967;
    PubMedGoogle Scholar
  • 3 Duckert F. The prephase accelerator. Present Status. Thrombos. Diathes. haemorrh. (Stuttg) 06: 244 1961;
    PubMedGoogle Scholar
  • 4 Schiffman S, Rapaport S. I, Patch M. J. The identification and synthesis of activated plasma thromboplastin component (PTC). Blood 22: 733 1963;
    PubMedGoogle Scholar
  • 5 Dodds W. J. Current concepts of hereditary coagulation disorders in dogs. Experimentation Animale 01: 243 1968;
    PubMedGoogle Scholar
  • 6 Mustard J. F, Roswell II C., Robinson G. A, Iloeksema T. D, Downie H. G. Canine haemophilia B (Christmas disease, P.T.C deficiency). Brit. J. Haemat 06: 259 1960;
    CrossrefPubMedGoogle Scholar
  • 7 Aggeler P. M, Hoag M. S, Kropatkin M. L, Kaplan S. S. Problems involved in the measurement of Factor IX: Physiological and Clinical implications, “The Hemophilias”. Brinkhous K. M. Ed. Univ. North Carolina Press; Chapel Hill: 1964
    Google Scholar
  • 8 Loeliger E. A, Hensen A, Mattem M. J, Veitkamp J. J, Brüning P. F, Hemker H. C. Treatment of Hemophilia B with purified Factor IX (PPSB). Foliamed. neerl 10: 112 1967;
    PubMedGoogle Scholar
  • 9 Yin E. T, Duckert F. The formation of intermediate product I in a purified system. The role of Factor IX or of its precursor and or a Hageman Factor-PTA fraction. Thrombos. Diathes. haemorrh. (Stuttg) 06: 224 1961;
    PubMedGoogle Scholar
  • 10 Nossel H. L. The Contact Phase of Blood Coagulation. Blackwell Scientific Publications; Oxford: 1964
    Google Scholar
  • 11 Lundblad R. L, Davies E. W. The activation of antihemophilic factor (Factor VIII) by activated Christmas Factor (Factor IX). Biochemistry 03: 1720 1964;
    CrossrefPubMedGoogle Scholar
  • 12 Biggs R, Macfarlane R. G, Denson K. W. E, Ash B. J. Thrombin and the Interaction of Factors VIII and IX. Brit. J. Haemat 11: 276 1965;
    CrossrefPubMedGoogle Scholar