Reaction Characteristics of Antiprothrombin Antibody on the Activation of Prothrombin (Sheep)^[*]

Summary

1. Prothrombin was purified by a series of steps and subsequently on DEAE-Cellu- lose chromatography and specific antibody to pure prothrombin was prepared.

2. The effect of Anti-prothrombin antibody was tested on the activation of prothrombin. The generated thrombin was assayed for its clotting function as well as for esterase activity on TAMe (Tosyl-Arginine-Methyl ester) as substrate.

3. Anti-prothrombin antibody inhibits the clotting function of generated thrombin completely but its esterase function is only partially affected.

^* Preliminary work was presented at the XIII Congress of the International Society of Hematology, Munich, 1970.

^** Present address : Department of Immunochemistry, Indian Statistical Institute, 203, B. T. Road, Calcutta - 35, India.

^*** Present address: Department of Medicine, University of Nebraska College of Medicine, Omaha, Nebraska U. S. A.

• References

• 1 Cebra J.John. (Personal communication).
• 2 Hicks N. D, Pitney W. R. A rapid screening test for disorders of thromboplastin generation. 1957
• 3 Hussain Q. Z, Newcomb T. F. Preparation of water insoluble thrombin. Proc. Soc. exp. Biol. (N. Y) 115: 301 1964;
• 4 Josso F, Lavergne J. M, Weilland Collete, Soulier J. F. Preparation of Human Prothrombin antibody. Thrombos. Diathes. haemorrh. (Stuttg) 18: 311 1967;
• 5 Mancini G, Veerman J. P, Carbonara A. O, Heremans J. P. A single radial diffusion method for the immunological quantitation of proteins. In: Protides Biological Fluids, 11th. Colloqn. Bruges. editor Peeters H. 370 1964
• 6 Newcomb T. F. (Personal Communication).
• 7 Ouchterlony O. Diffusion in gel methods for the immunological analysis, in : Progress in Allergy. 06: 30 1962;
• 8 Owren P. A. Diagnostic and prognostic significance of plasma prothrombin and factor V levels in parenchymatous hepatitis and obstructive jaundice. Scand. J. clin. Lab. Invest 01: 131 1949;
• 9 Quick A. J. Hemorrhagic Diseases. Academic press; New York, USA: 1959
• 10 Robbins J. B, Haimonich J, Sela M. Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose. Immunochemistry 04: 11 1967;
• 11 Seegers W. M, Murano G, McCoy L. 1970
• 12 Shapiro.
• 13 Sigler P. S, Blow D. M, Matthews B. W, Henderson R. Structure of crystalline X-Chymo- trypsin II. A preliminary report including a Hypothesis for the activation mechanism. J. Mol. Biol 35: 143 1968;
• 14 Stormorken H. Preparation of proaccelerin deficient (parahemophilia) plasma for the assay of proaccelerin. Scand. J. clin. Lab. Invest 09: 973 1957;