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Thromb Haemost 1977; 37(02): 321-328
DOI: 10.1055/s-0038-1649232
Original Article
Schattauer GmbH

Binding of Anti-Actin Autoantibodies to Platelets

Claude A Bouvier
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
,
Giulio Gabbiani
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
,
Graeme B Ryan
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
,
Marie-Claude Badonnel
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
,
Guido Majno
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
,
Ernst F Lüscher
1   Unité d’Hémostase, Hôpital Cantonal, Geneva, Department of Pathology, University of Geneva, and Theodor Kocher Institute, University of Bern, Switzerland
› Author Affiliations
Further Information

Publication History

Received 14 September 1976

Accepted 30 November 1976

Publication Date:
03 July 2018 (online)

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Summary

Normal platelets incubated with anti-actin autoantibodies (AAA) (from the serum of patients with chronic aggressive hepatitis) do not show binding of these antibodies as seen by indirect immunofluorescence. AAA serum does not inhibit thrombin-induced clot retraction, despite the binding of the antibodies to platelets in the clot. Similarly, AAA serum does not affect “reversible” or “irreversible” aggregation (induced by ADP, collagen or epinephrine), despite the binding of the antibodies to platelet actin under such circumstances. AAA also bind to platelets when aggregation is inhibited by EDTA. The incubation of “reversibly” aggregated platelet with AAA results in a small but definite binding of AAA to platelets. These findings suggest that during “irreversible” and/or “reversible” aggregation, changes take place at the surface of platelets which expose the antigen at the surface of the cell.

 

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