Thromb Haemost 1977; 37(01): 123-135
DOI: 10.1055/s-0038-1649210
Original Article
Schattauer GmbH

The Reversible Antithrombin Activity of Incubated Plasma

E. D Gomperts
1  Department of Haematology, Royal Postgraduate Medical School, Ducane Road, London W12, England.
,
J. C Stavridis
1  Department of Haematology, Royal Postgraduate Medical School, Ducane Road, London W12, England.
,
R. S Mibashan
1  Department of Haematology, Royal Postgraduate Medical School, Ducane Road, London W12, England.
› Author Affiliations
Further Information

Publication History

Received 12 January 1976

Accepted 03 September 1976

Publication Date:
03 July 2018 (online)

Summary

The thrombin time of normal citrated plasma is progressively prolonged on incubation in open glass tubes at 37° C. The phenomenon is dependent on the temperature and duration of incubation, on the pH, and on the concentration of calcium ions present. Platelet-rich citrated plasma fails to exhibit augmented antithrombin activity when similarly incubated, and the addition of washed platelets to platelet-poor plasma inhibits this activity. The clot retarding action of incubated plasma against thrombin is also manifested against Arvin (Ancrod), but not against Reptilase-R. This thrombin time lenghening may be inhibited by incubation with anti-antithrombin III antiserum thus indicating that the phenomenon of thrombin time lengthening is consistent with enhanced activity of antithrombin III. It is unlikely that alterations in the activity of α2-macroglobulin, is important in the reduced thrombin-coagulability of incubated plasma. Interference with the polymerisation of fibrin monomers by the physicochemical changes may contribute to the observed phenomenon.