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Thromb Haemost 1974; 31(03): 485-492
DOI: 10.1055/s-0038-1649188
Original Article
Schattauer GmbH

Heterogeneity of Rabbit Platelets. I. Employment of an Albumin Density Gradient for Separation of a Young Platelet Population Identified with Se75-Selenomethionine

Arthur Charmatz
1   Department of Medicine, New York University Medical School, 550 First Avenue, New York, N. Y. 10016 U.S.A.
,
Simon Karpatkin
1   Department of Medicine, New York University Medical School, 550 First Avenue, New York, N. Y. 10016 U.S.A.
› Author Affiliations
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Publication History

Received 31 January 1974

Accepted 27 February 1974

Publication Date:
29 June 2018 (online)

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Summary

An albumin density gradient was employed to separate rabbit platelets into heavy and light populations. Compared to a previous inert oil density gradient, the albumin gradient had the advantage of greater resolution, easier suspension of isolated platelet populations, and more physiologic composition. Heavy platelets were found to be enriched with large platelets, containing 2.3 fold more platelets with 2 μ diameter and 2 fold less platelets with 1 μ diameter. Platelets of 3 and 4 μ diameters were found in the heavy population but not in the light population. In vivo labelling of megakaryocytes with Se75-selenomethionine revealed early preferential labelling of heavy platelets when compared to light platelets, with an average heavy/light ratio labelling index of 3.7 on days 1–2, declining to 1.4 on days 3–4 and 0.7 on day 6. These data support previous data that heavy-large platelets are young platelets.