Thromb Haemost 1974; 31(02): 309-318
DOI: 10.1055/s-0038-1649165
Original Article
Schattauer GmbH

The Effects of Amines on the Esterase Activities of Thrombin and Thrombokinase and on Several Clotting Tests[*]

Phyllis S Roberts
1   Division of Medical Oncology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond Virginia, 23298, U8A
,
Raphael M Ottenbrite
1   Division of Medical Oncology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond Virginia, 23298, U8A
,
Patricia B Fleming
1   Division of Medical Oncology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond Virginia, 23298, U8A
,
James Wigand
1   Division of Medical Oncology, Department of Medicine, Medical College of Virginia, Virginia Commonwealth University, Richmond Virginia, 23298, U8A
› Author Affiliations
Further Information

Publication History

Received 24 July 1973

Accepted 31 December 1973

Publication Date:
29 June 2018 (online)

Summary

1. Choline chloride, 0.1 M (in 0.25 M Tris. HCl buffer, pH 7.4 or 8.0, 37°), doubles the rate of hydrolysis of TAME by bovine thrombokinase but has no effect on the hydrolysis of this ester by either human or bovine thrombin. Only when 1.0 M or more choline chloride is present is the hydrolysis of BAME by thrombokinase or thrombin weakly inhibited. Evidence is presented that shows that these effects are due to the quaternary amine group.

2. Tetramethyl ammonium bromide or chloride has about the same effects on the hydrolysis of esters by these enzymes as does choline chloride but tetra-ethyl, -n.propyl and -n.butyl ammonium bromides (0.1 M) are stronger accelerators of the thrombokinase-TAME reaction and they also accelerate, but to a lesser degree, the thrombin-TAME reaction. In addition, they inhibit the hydrolysis of BAME by both enzymes. Their effects on these reactions, however, do not follow any regular order. The tetraethyl compound is the strongest accelerator of the thrombokinase-TAME reaction but the tetra-ethyl and -butyl compounds are the strongest accelerators of the thrombin-TAME reaction. The ethyl and propyl compounds are the best (although weak) inhibitors of the thrombokinase-BAME and the propyl compound of the thrombin-BAME reactions.

3. Tetra-methyl, -ethyl, -n.propyl and -n.butyl ammonium bromides (0.01 M) inhibit the clotting of fibrinogen by thrombin (bovine and human proteins) at pH 7.4, imidazole or pH 6.1, phosphate buffers and they also inhibit, but to a lesser degree, a modified one-stage prothrombin test. In all cases the inhibition increases regularly as the size of the alkyl group increases from methyl to butyl. Only the ethyl com pound (0.025 M but not 0.01 M), however, significantly inhibits the polymerization of bovine fibrin monomers. It was concluded that inhibition of the fibrinogen-thrombin and the one-stage tests by the quaternary amines is not due to any effect of the com pounds on the polymerization process but probably due to inhibition of thrombin’s action on fibrinogen by the quaternary amines.

* Presented at the IVth International Congress on Thrombosis and Haemostasis, Vienna, Austria, June 19–22, 1973.


 
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