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Thromb Haemost 1974; 31(01): 072-085
DOI: 10.1055/s-0038-1649147
Original Article
Schattauer GmbH

Isolation and Characterization of Plasminogen Activator from Pig Leucocytes

M Kopitar
1   Department of Biochemistry, J. Stefan Institute, University of Ljubljana and Institute of Gerontology, IIIrd Clinic of Medicine, Ljubljana, Yugoslavia
,
M Stegnar
1   Department of Biochemistry, J. Stefan Institute, University of Ljubljana and Institute of Gerontology, IIIrd Clinic of Medicine, Ljubljana, Yugoslavia
,
B Accetto
1   Department of Biochemistry, J. Stefan Institute, University of Ljubljana and Institute of Gerontology, IIIrd Clinic of Medicine, Ljubljana, Yugoslavia
,
D Lebez
1   Department of Biochemistry, J. Stefan Institute, University of Ljubljana and Institute of Gerontology, IIIrd Clinic of Medicine, Ljubljana, Yugoslavia
› Author Affiliations
Further Information

Publication History

Received 18 July 1973

Accepted 20 October 1973

Publication Date:
29 June 2018 (online)

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Summary

Plasminogen activator was isolated from disrupted pig leucocytes by the aid of DEAE chromatography, gel filtration on Sephadex G-100 and final purification on CM cellulose, or by preparative gel electrophoresis.

Isolated plasminogen activator corresponds No. 3 band of the starting sample of leucocyte cells (that is composed from 10 gel electrophoretic bands).

pH optimum was found to be in pH range 8.0–8.5 and the highest pH stability is between pH range 5.0–8.0.

Inhibition studies of isolated plasminogen activator were performed with EACA, AMCHA, PAMBA and Trasylol, using Anson and Astrup method. By Astrup method 100% inhibition was found with EACA and Trasylol and 30% with AMCHA. PAMBA gave 60% inhibition already at concentration 10–3 M/ml. Molecular weight of plasminogen activator was determined by gel filtration on Sephadex G-100. The value obtained from 4 different samples was found to be 28000–30500.

 

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