The Corrective Effect of Factor VIII-coated Red Blood Cells in Haemophilic Plasma with Antibodies to Factor VIII

Summary

Factor VIII-coated red blood cells had an evident corrective effect on plasma of haemophiliacs with inhibitors. This effect was especially significant when tanned coated red cells were used. The direct coating of washed red blood cells by factor VIII concentrates is also feasible. The results were better with fraction C-I (AHG concentrate containing dextran) than with fibrinogen-free concentrate. It seems that dextran enhances the attachment of factor VIII to human erythrocytes. In the direct coating-procedure the time of incubation was important, but temperature and pH were not critical. The results obtained indicate that factor VIII attached to red blood cells is more resistant to inhibitors than factor VIII in solution.

The possibility of using factor VIII-coated red cells for the management of patients with antibodies to factor VIII is discussed.

• References

• 1 Biggs B, Bidwell E. A method for the study of antihaemophilic globulin inhibitors with reference to six cases. British Journal of Haematology 1959; 5: 379
• 2 Herbert W. J. Passive haemagglutination. In Weir D. W. (ed.) Handbook of Experimental Immunology Blackwell Scientific Publications; Oxford and Edinburgh: 1967: 720
• 3 łopaciuk S, Naeeczyńska A, Daszyński J, Klenowska Z. Nowy koncentrat globuliny przeciwhemofilowej (czynnika VIII) otrzymywany metoda krioprecypitacji i przy uzyciu dextranu. Acta Hlaemat 1971; Pol. 2: 103
• 4 łopaciue S, Latałło Z. S. Separation of human antihaemophilic factor (AHF, Factor VIII) from fibrinogen by means of Defibrase (Beptilase). IVth International Congress on Thrombosis and Haemostasis; Vienna, Austria: 1973: 1973
• 5 Rizza C. B. The management of haemophilia complicated by factor VIII antibodies. In: Biggs B. (ed.) Human Blood Coagulation Haemostasis and Thrombosis Blackwell Scientific Publications; Oxford: 1972: 343
• 6 Stites O. P, Hershgold E. J, Perlman J. D, Fudenberg H. H. Factor VIII detection by haemagglutination inhibition: Hemophilia A and von Willebrand’s disease. Science 1971; 171: 196
• 7 Uszyński L. unpublished observation