Thromb Haemost 1974; 31(01): 003-011
DOI: 10.1055/s-0038-1649140
Original Article
Schattauer GmbH

Factor VIII International Units and Reference Materials

Derek R Bangham
1   National Institute for Biological Standards and Control, Holly Hill, Hamp-stead, London NW 3 6 RB, England
,
Milica Brozović
1   National Institute for Biological Standards and Control, Holly Hill, Hamp-stead, London NW 3 6 RB, England
› Author Affiliations
Further Information

Publication History

Received 04 November 1973

Accepted 06 November 1973

Publication Date:
29 June 2018 (online)

Summary

The establishment of the International Standard for factor VIII has introduced a common yardstick, the international unit, which all countries can apply. This standard is a concentrate of intermediate purity, shown to be very stable on long term storage. As with all international standards it is available in limited quantities and is primarily used for the calibration of national standards used to monitor patients and in the control of therapeutic preparations.

Since 1969 in Great Britain such a national standard has been made from freeze-dried pooled plasma. This has been available in larger quantities and has been introduced into routine use in coagulation laboratories. As factor VIII in freeze-dried plasma has limited stability even when stored at —20° C, successive new batches are prepared every two years or so. The availability of a common working standard with factor VIII content specified in international units makes it possible to define diagnostic and therapeutic criteria with more precision than hitherto. This system can be applied in other countries and to other clotting factors for which an international yardstick is needed.

 
  • References

  • 1 Bangham D. R, Biggs R, Brozović M, Denson K. W. E, Skegg J. L. A biological standard for measurement of blood coagulation factor VIII activity. Bulletin of the World Health Organization 1971; 45: 331
  • 2 Bangham D. R, Brozović M, Robertson I. Stability of some clotting factors in freeze dried factor VIII reference plasma and concentrate. Haemophilia.: VIth Congress of the World Federation of Haemophilia. Baden, 25-27 July 1970, 1971: 89
  • 3 British Pharmacopoeia Dried Human Antihaemophilic Fraction, Her Majesty’s Stationary Office London 1973. 1973: 64
  • 4 Britten A, Grove-Rasmussen M. Stability of factor VIII in the frozen state. Transfusion 1966; 6: 230
  • 5 Brozović M, Gurd L. J, Robertson I, Bangham D. R. Factor X in freeze dried factor VIII reference plasma. British Journal of Haematology 1971; a 21: 201
  • 6 Brozović M, Gurd L. J, Robertson I, Bangham D. R. Stability of prothrombin and factor VIII in freeze dried plasma. Journal of Clinical Pathology 1971; b 24: 690
  • 7 Brozović M, Stirling Y, Harricks C, North W. R. C, Mead T. W. Coagulation factors in an industrial population. I. Factor VIII. To be published 1973
  • 8 Denson K. W. E. Standardisation of methods for the determination of antihaemophilic factors. Proceedings of the VIth Congress of the World Federation of Haemophilia 1970. Baden, 25-27 July 82
  • 9 Denson K. W. E, Biggs R. Laboratory diagnosis, tests of clotting function and their standardisation. In: Human Blood Coagulation, Haemostasis and Thrombosis Blackwell Scientific Publications; Oxford, London, Edinburgh, Melbourne: 1972: 295
  • 10 Hills M, Ingram G. I. C, Matchett M. Lyophilized plasma as assay standard for factor VIII: calibration and control with fresh plasma samples. British Journal of Haematology 1970; 18: 617
  • 11 Ingram G. I. C. Blood coagulation factor VIII: Genetics, physiological control and bioassay. Advances in Clinical Chemistry 1965; 8: 189
  • 12 Jerne N. K, Perry W. L. M. The stability of biological standards. Bulletin of the World Health Organization 1955; 14: 167
  • 13 Newman J, Johnson A. J, Karpatkin M. H, Puszkin S. Method for the production of high purity factor VIII concentrate. British Journal of Haematology 1971; 21: 1
  • 14 Preston A. E. The plasma concentration of factor VIII in the normal population. 1. Mothers and babies at birth. British Journal of Haematology 1964; 10: 110
  • 15 Preston A. E, Barr A. The plasma concentration of factor VIII in the normal population: II. The effects of age. sex and blood group. British Journal of Haematology 1964; 10: 238
  • 16 Preston A. E. The factor VIII activity in fresh and stored plasma. British Journal of Haematology 1967; 13: 42
  • 17 Spaet T. H, Garner E. S. Studies on the storage lability of human antihaemophilic factor. Journal of Laboratory and Clinical Medicine 1955; 46: 111
  • 18 Thelin G. M. Preparation and standardisation of a stable AHF plasma. Thrombosis et Diathesis Haemorrhagica 1968; 19: 423
  • 19 Weisert O, Jeremic M. Preservation of coagulation factors V and VIII during collection and subsequent storage of bank blood in ACD A and CPD solutions. Vox Sang 1973; 24: 126
  • 20 World Health Organization Technical Reports Series 1971; 463: 14
  • 21 Zucker S, Cathey M, West B. Preparation of quality control speciments for coagulation. American Journal of Clinical Pathology 1970; 53: 924